BindingDB PrimarySearch

BDBM2583 3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione::3,4-bis(1H-indol-3-yl)-2,5-dihydro-1H-pyrrole-2,5-dione::BIM-4::Bisindolyl deriv. 3::Bisindolylmaleimide deriv. 5::CHEMBL266487::bis-indolylmaleimide deriv. 8a

SMILES: O=C1NC(=O)C(=C1c1c[nH]c2ccccc12)c1c[nH]c2ccccc12

InChI Key: InChIKey=DQYBRTASHMYDJG-UHFFFAOYSA-N

Data: 18 IC50 1 Kd 1 ITC

PDB links: 9 PDB IDs contain this monomer as substructures.

Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB

Found 19 hits for monomerid = 2583
Target/Host (Institution)	Ligand	Target/Host Links	Ligand Links	Trg + Lig Links	Ki nM	ΔG° kcal/mole	IC50 nM	Kd nM	EC50/IC50 nM	k_off s^-1	k_on M^-1s^-1	pH	Temp °C
Protein Kinase C (Rattus norvegicus (rat))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB UniProtKB/SwissProt GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	550	n/a	n/a	n/a	n/a	7.5	30
Roche Products Limited					Assay Description The activity of PKC, activated by phosphatidylerine and Ca2+, is measured by its ability to transfer phosphate from [gamma-32P]ATP to lysine-rich his...				J Med Chem 35: 177-84 (1992) Article DOI: 10.1021/jm00079a024 BindingDB Entry DOI: 10.7270/Q2K64G8V
Roche Products Limited					More data for this Ligand-Target Pair
Cyclin B/Cyclin-Dependent Kinase 1 (CDK1)/G2/mitotic-specific cyclin B2/G2/mitotic-specific cyclin B3 (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB UniProtKB/SwissProt antibodypedia antibodypedia antibodypedia antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	PubMed	n/a	n/a	1.80E+4	n/a	n/a	n/a	n/a	n/a	n/a
Kyushu University Curated by ChEMBL					Assay Description Inhibitory concentration was determined by measuring phoshporylation of H1 histone using active human Cell division cycle 2-cyclin B complex				Bioorg Med Chem Lett 8: 1019-22 (1999) BindingDB Entry DOI: 10.7270/Q27W6BBN
Kyushu University Curated by ChEMBL					More data for this Ligand-Target Pair
cAMP-Dependent Protein Kinase (PKA) (Bos taurus (bovine))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB UniProtKB/SwissProt GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	PubMed	n/a	n/a	100	n/a	n/a	n/a	n/a	7.4	30
Laboratoires Glaxo					Assay Description IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labe...				J Biol Chem 266: 15771-81 (1991) BindingDB Entry DOI: 10.7270/Q2FF3QJ4
Laboratoires Glaxo					More data for this Ligand-Target Pair
cAMP-Dependent Protein Kinase (PKA) (Rattus norvegicus (rat))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB Reactome pathway UniProtKB/SwissProt B.MOAD GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	PubMed	n/a	n/a	2.00E+3	n/a	n/a	n/a	n/a	7.0	30
Laboratoires Glaxo					Assay Description IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labe...				J Biol Chem 266: 15771-81 (1991) BindingDB Entry DOI: 10.7270/Q2FF3QJ4
Laboratoires Glaxo					More data for this Ligand-Target Pair
Phosphorylase Kinase (Oryctolagus cuniculus (rabbit))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	UniProtKB/SwissProt GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	PubMed	n/a	n/a	3.20E+3	n/a	n/a	n/a	n/a	7.0	30
Laboratoires Glaxo					Assay Description IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labe...				J Biol Chem 266: 15771-81 (1991) BindingDB Entry DOI: 10.7270/Q2FF3QJ4
Laboratoires Glaxo					More data for this Ligand-Target Pair
Cyclin-Dependent Kinase 4 (CDK4) (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB NCI pathway Reactome pathway KEGG UniProtKB/SwissProt UniProtKB/TrEMBL B.MOAD DrugBank antibodypedia antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	5.81E+3	n/a	n/a	n/a	n/a	7.0	22
DCR&T, Lilly Spain S.A.					Assay Description In vitro CDK assay using purified enzyme, was incubated at room temperature with substrate, and test compounds in the presence of ATP/[gamma-33P]ATP....				Bioorg Med Chem Lett 13: 3841-6 (2003) Article DOI: 10.1016/S0960-894X(03)00792-3 BindingDB Entry DOI: 10.7270/Q2FN14C5
DCR&T, Lilly Spain S.A.					More data for this Ligand-Target Pair
CDK2/CycE (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB UniProtKB/SwissProt UniProtKB/TrEMBL antibodypedia antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	1.17E+4	n/a	n/a	n/a	n/a	n/a	25
DCR&T, Lilly Spain S.A.					Assay Description In vitro CDK assay using purified enzyme, was incubated at room temperature with substrate, and test compounds in the presence of ATP/[gamma-33P]ATP....				Bioorg Med Chem Lett 13: 3841-6 (2003) Article DOI: 10.1016/S0960-894X(03)00792-3 BindingDB Entry DOI: 10.7270/Q2FN14C5
DCR&T, Lilly Spain S.A.					More data for this Ligand-Target Pair
cAMP-Dependent Protein Kinase (PKA) (Bos taurus (bovine))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB UniProtKB/SwissProt GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	>2.00E+4	n/a	n/a	n/a	n/a	n/a	25
DCR&T, Lilly Spain S.A.					Assay Description In vitro kinase assay using purified enzyme, was incubated at room temperature with substrate, and test compounds in the presence of ATP/[gamma-33P]A...				Bioorg Med Chem Lett 13: 3841-6 (2003) Article DOI: 10.1016/S0960-894X(03)00792-3 BindingDB Entry DOI: 10.7270/Q2FN14C5
DCR&T, Lilly Spain S.A.					More data for this Ligand-Target Pair
Epidermal growth factor receptor (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB KEGG UniProtKB/SwissProt B.MOAD DrugBank antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	PubMed	n/a	n/a	>5.00E+4	n/a	n/a	n/a	n/a	n/a	n/a
Ciba-Geigy Limited Curated by ChEMBL					Assay Description Inhibitory activity against recombinant tyrosine kinase EGF-R (EGF-R ICD)				J Med Chem 37: 1015-27 (1994) BindingDB Entry DOI: 10.7270/Q2M32TTP
Ciba-Geigy Limited Curated by ChEMBL					More data for this Ligand-Target Pair
Cyclic AMP-dependent protein kinase (PKA) (Oryctolagus cuniculus (Rabbit))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB KEGG UniProtKB/TrEMBL B.MOAD GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	PubMed	n/a	n/a	2.00E+5	n/a	n/a	n/a	n/a	n/a	n/a
Ciba-Geigy Limited Curated by ChEMBL					Assay Description Inhibitory activity was determined against protein kinase A (PKA) of rabbit				J Med Chem 37: 1015-27 (1994) BindingDB Entry DOI: 10.7270/Q2M32TTP
Ciba-Geigy Limited Curated by ChEMBL					More data for this Ligand-Target Pair
Tyrosine-protein kinase ABL (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB KEGG UniProtKB/SwissProt UniProtKB/TrEMBL B.MOAD DrugBank antibodypedia antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	PubMed	n/a	n/a	2.00E+4	n/a	n/a	n/a	n/a	n/a	n/a
Ciba-Geigy Limited Curated by ChEMBL					Assay Description Inhibitory activity against v-abl tyrosine kinase				J Med Chem 37: 1015-27 (1994) BindingDB Entry DOI: 10.7270/Q2M32TTP
Ciba-Geigy Limited Curated by ChEMBL					More data for this Ligand-Target Pair
Serine/threonine-protein kinase pim-1 (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB KEGG UniProtKB/SwissProt UniProtKB/TrEMBL B.MOAD DrugBank antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	n/a	27	n/a	n/a	n/a	n/a	n/a
Oxford University Curated by ChEMBL					Assay Description Binding affinity to non phosphorylated PIM1				J Med Chem 48: 7604-14 (2005) Article DOI: 10.1021/jm0504858 BindingDB Entry DOI: 10.7270/Q2Q52R4H
Oxford University Curated by ChEMBL					More data for this Ligand-Target Pair
PKC alpha and beta-2 (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB NCI pathway Reactome pathway KEGG UniProtKB/SwissProt B.MOAD DrugBank antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	212	n/a	n/a	n/a	n/a	n/a	n/a
Central Pharmaceutical Research Institute Curated by ChEMBL					Assay Description Inhibitory concentration against recombinant human Protein kinase C beta 2				Bioorg Med Chem Lett 14: 5171-4 (2004) Article DOI: 10.1016/j.bmcl.2004.07.061 BindingDB Entry DOI: 10.7270/Q2K35VDJ
					More data for this Ligand-Target Pair
Protein kinase C alpha type (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB NCI pathway Reactome pathway KEGG UniProtKB/SwissProt UniProtKB/TrEMBL B.MOAD DrugBank antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	1.60E+3	n/a	n/a	n/a	n/a	n/a	n/a
Central Pharmaceutical Research Institute Curated by ChEMBL					Assay Description Inhibitory concentration against recombinant human Protein kinase C alpha				Bioorg Med Chem Lett 14: 5171-4 (2004) Article DOI: 10.1016/j.bmcl.2004.07.061 BindingDB Entry DOI: 10.7270/Q2K35VDJ
					More data for this Ligand-Target Pair
NAD-dependent deacetylase sirtuin 2 (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB NCI pathway KEGG UniProtKB/SwissProt B.MOAD antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	4.70E+3	n/a	n/a	n/a	n/a	n/a	n/a
Albert-Ludwigs-Universität Freiburg Curated by ChEMBL					Assay Description Inhibition of human recombinant SIRT2				J Med Chem 49: 7307-16 (2006) Article DOI: 10.1021/jm060118b BindingDB Entry DOI: 10.7270/Q2TQ616P
Albert-Ludwigs-Universität Freiburg Curated by ChEMBL					More data for this Ligand-Target Pair
PKC alpha and beta-2 (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB NCI pathway Reactome pathway KEGG UniProtKB/SwissProt B.MOAD DrugBank antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	304	n/a	n/a	n/a	n/a	n/a	n/a
Central Pharmaceutical Research Institute Curated by ChEMBL					Assay Description Inhibitory concentration against recombinant human Protein kinase C beta 1				Bioorg Med Chem Lett 14: 5171-4 (2004) Article DOI: 10.1016/j.bmcl.2004.07.061 BindingDB Entry DOI: 10.7270/Q2K35VDJ
					More data for this Ligand-Target Pair
Protein kinase C, epsilon (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB MMDB NCI pathway Reactome pathway KEGG UniProtKB/SwissProt UniProtKB/TrEMBL B.MOAD DrugBank antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	7.52E+3	n/a	n/a	n/a	n/a	n/a	n/a
Central Pharmaceutical Research Institute Curated by ChEMBL					Assay Description Inhibitory concentration against recombinant human Protein kinase C epsilon				Bioorg Med Chem Lett 14: 5171-4 (2004) Article DOI: 10.1016/j.bmcl.2004.07.061 BindingDB Entry DOI: 10.7270/Q2K35VDJ
					More data for this Ligand-Target Pair
Calcium/calmodulin-dependent protein kinase type II subunit delta (Homo sapiens (Human))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB KEGG UniProtKB/SwissProt B.MOAD antibodypedia GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	1.19E+3	n/a	n/a	n/a	n/a	n/a	n/a
Scios, Inc. Curated by ChEMBL					Assay Description Inhibition of CaMK2delta				Bioorg Med Chem Lett 18: 2395-8 (2008) Article DOI: 10.1016/j.bmcl.2008.02.058 BindingDB Entry DOI: 10.7270/Q2QZ2BVW
Scios, Inc. Curated by ChEMBL					More data for this Ligand-Target Pair
cAMP-Dependent Protein Kinase (PKA) (Bos taurus (bovine))	BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...) Show SMILES Show InChI	PDB UniProtKB/SwissProt GoogleScholar AffyNet	Purchase CHEMBL PC cid PC sid UniChem Patents Similars	Article PubMed	n/a	n/a	1.18E+4	n/a	n/a	n/a	n/a	8.5	37
Roche Products Limited					Assay Description The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.				J Med Chem 35: 177-84 (1992) Article DOI: 10.1021/jm00079a024 BindingDB Entry DOI: 10.7270/Q2K64G8V
Roche Products Limited					More data for this Ligand-Target Pair

Activity Spreadsheet -- ITC Data from BindingDB

Found 1 hit for monomerid = 2583
Cell (A)	Syringe (B)	Cell Links	Syringe Links	Cell + Syr Links	ΔG° kcal/mole	-TΔS° kcal/mole	ΔH° kcal/mole	log K	pH	Temp °C
BDBM2583 (3,4-Bis(3-indolyl)-1H-pyrrole-2,5-dione \| 3,4-bis(...)	Serine/threonine-protein kinase PIM (Homo sapiens (Human))	PC cid PC sid	DrugBank GoogleScholar KEGG PDB		-9.78	0.548	-10.4	7.57	7.5	10
	Oxford University	PC cid PC sid	DrugBank GoogleScholar KEGG PDB		J Med Chem 48: 7604-14 (2005)