Abstract
[(3)H]BBL454, a new CCK(2) selective tritiated agonist was prepared via the reductive tritiation of a 5-aminopentyn-1-yl moiety introduced on the N-terminal end of a pentapeptide derivative of cholecystokinin. The binding properties of this labelled compound were determined on CHO cells transfected with the rat CCK(2) receptor. [(3)H]BBL454 is able to discriminate two affinity states of the CCK(2) receptor a supplementary indication of its validity for further exploring the heterogeneity of this receptor.
MeSH terms
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Animals
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CHO Cells
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Cholecystokinin / analogs & derivatives
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Cholecystokinin / chemical synthesis*
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Cholecystokinin / metabolism*
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Cricetinae
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Guinea Pigs
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Oligopeptides / chemical synthesis*
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Oligopeptides / metabolism
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Peptide Fragments / chemical synthesis*
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Peptide Fragments / metabolism*
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Radioligand Assay / methods
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Rats
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Receptor, Cholecystokinin B / agonists*
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Receptor, Cholecystokinin B / metabolism
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Tritium / metabolism
Substances
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(3H)BBL454, peptide
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Oligopeptides
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Peptide Fragments
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Receptor, Cholecystokinin B
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Tritium
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Cholecystokinin