GPR4 antagonist 1

Name: GPR4 antagonist 1
Brand: MedChemExpress
SKU: HY-101536
Rating: 4.5 (1 reviews)

Cat. No.: HY-101536 Purity: 98.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

GPR4 antagonist 1 is a GPR4 antagonist, with an IC₅₀ of 189 nM.

For research use only. We do not sell to patients.

GPR4 antagonist 1 Chemical Structure

CAS No. : 1197879-16-5

Size	Price	Stock	Quantity
Oil + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 638	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 638	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 920	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1850	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3000	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4800	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

GPR4 antagonist 1 is a GPR4 antagonist, with an IC₅₀ of 189 nM.

IC₅₀ & Target

IC50: 189 nM (GPR4)^[1].

Molecular Weight

431.62

Formula

C₂₇H₃₇N₅

CAS No.

1197879-16-5

Appearance

Oil

Color

Light yellow to yellow

SMILES

CC1=C2C(N(CC3=CC=C(/C=C/CN4CCN(C(C)C)CC4)C=C3)C(CC)=N2)=NC(C)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (289.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3169 mL	11.5843 mL	23.1685 mL
5 mM	0.4634 mL	2.3169 mL	4.6337 mL
10 mM	0.2317 mL	1.1584 mL	2.3169 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.82 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.92%

Select Batch:

Data Sheet (268 KB) SDS (252 KB)

COA (245 KB) HNMR (269 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. ARYL SULFONOHYDRAZIDES. US 20090291942 A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3169 mL	11.5843 mL	23.1685 mL	57.9213 mL
	5 mM	0.4634 mL	2.3169 mL	4.6337 mL	11.5843 mL
	10 mM	0.2317 mL	1.1584 mL	2.3169 mL	5.7921 mL
	15 mM	0.1545 mL	0.7723 mL	1.5446 mL	3.8614 mL
	20 mM	0.1158 mL	0.5792 mL	1.1584 mL	2.8961 mL
	25 mM	0.0927 mL	0.4634 mL	0.9267 mL	2.3169 mL
	30 mM	0.0772 mL	0.3861 mL	0.7723 mL	1.9307 mL
	40 mM	0.0579 mL	0.2896 mL	0.5792 mL	1.4480 mL
	50 mM	0.0463 mL	0.2317 mL	0.4634 mL	1.1584 mL
	60 mM	0.0386 mL	0.1931 mL	0.3861 mL	0.9654 mL
	80 mM	0.0290 mL	0.1448 mL	0.2896 mL	0.7240 mL
	100 mM	0.0232 mL	0.1158 mL	0.2317 mL	0.5792 mL