new BindingDB logo
myBDB logout
Solution Informationhelp
Enzyme: Glycogen synthase kinase-3 beta
inhibitor: BDBM32102
substrate: n/a
 
Solution Type: Aqueous
pH at Preparation: n/a
Temp. Prep.: n/a
Comments: 1) Dispense 1 uL/well of CABPE, 0.5 uL of ATP, and 1 uL of positive control GW8510 or AB in respective wells according to plate design to 1536-well assay ready plates (Aurora 29847) generated by acoustic transfer using Labcyte Echo that contain 7.5 nL/well of 10 mM compound using BioRAPTR (Beckman) to start the reaction. Incubate at room temperature for 60 minutes. 2) Add 2.5 uL/well of ADP-glo (Promega, V9103) with BioRAPTR, incubate at room temperature for 40 minutes 3) Add 5 uL/well of ADP-glo (Promega, V9103) with Combi nL (Thermo), incubate at room temperature for 30 minutes 4) Read on Viewlux (PerkinElmer) for luminescence Solutions: AB: 25 mM Tris 7.5 10 mM MgCl2 GW8510 (in AB, Sigma G7791) 50 uM GW8510 CABPE (in AB): 12.5 mM DTT (Sigma 43816) 0.25 mg/mL BSA (Sigma A4503) 0.5 U/mL Heparin (Baxter NDC 0641-2440-41) 8 uM Peptide (American Peptide) 9 nM GSK3beta (BPS Biosciences) ATP (in AB, Promega V9103 component): 125 uM ATP
 
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.