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Solution Informationhelp
Enzyme: Hematopoietic protein-tyrosine phosphatase (HEPTP)
inhibitor: BDBM34452
substrate: n/a
 
Solution Type: Aqueous
pH at Preparation: n/a
Temp. Prep.: n/a
Comments: HePTP assay materials: HePTP assay materials: 1)HePTP protein was provided by Dr. Mustelin (Sanford-Burnham Medical Research Institute, San Diego, CA). 2)Assay Buffer: 50 mM Bis-Tris, pH 6.0, 375 mM NaCl, 0 mM DTT, 0.0125% Tween 20. 3) Upon pre-incubation for 10 min. in the presence of 25 mM DTT, 20 uM HePTP enzyme stock was aliquoted and flash-frozen. HePTP working solution at 3 nM in the assay buffer without DTT was prepared using this pre-incubated stock. 4) OMFP working solution: 600 uM OMFP in the assay buffer HePTP dose-response assay protocol: 1) Using Thermo Scientific MultiDrop Combi dispense 10 ul of OMFP working solution into all columns of black small-volume Greiner plates (784076) 2) Using Thermo Scientific MultiDrop Combi dispense 10 uL of assay buffer in columns 1-2. 3) Using LabCyte Echo 550 add 0.2 uL of serially diluted compounds to columns 3-22 and DMSO to columns 1-2 and 23-24. Final concentration of DMSO was 1 % 4) Using Thermo Scientific MultiDrop Combi dispe
 
 

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Last update November 1, 2007
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