new BindingDB logo
myBDB logout
Solution Informationhelp
Enzyme: Tyrosine-protein phosphatase non-receptor type 22
inhibitor: BDBM28637
substrate: n/a
 
Solution Type: Aqueous
pH at Preparation: n/a
Temp. Prep.: n/a
Comments: Assay materials: 1) LYP (LYPCAT) was produced at the Sanford-Burnham Medical Research Institute's protein production core facility 2) Assay Buffer: 20mM Bis-Tris pH 6.0, 1mM DTT, 0.005% Tween-20 3) 3-O-Methylfluorescein phosphate (OMFP) cyclohexylammonium salt (Sigma-Aldrich, M2629) Dose response protocol: 1) Dispense 10 ul of 600 uM OMFP in assay buffer into all columns of black 384-well small-volume Greiner plates (786076) 2) Dispense 10 uL of assay buffer in columns 1-2. 3) Using Labcyte Echo550, dispense 200 nL of serially diluted compounds to columns 3-22 and DMSO to columns 1-2 and 23-24. Final concentration of DMSO was 1 % 4) Using Thermo Scientific MultiDrop Combi, dispense 10 uL of 3 nM LYPCAT working solution to wells in columns 3 through 24. 5) Incubate lidded plate for 30 min. at room temp. 6) Read plate on M5 plate reader in fluorescence mode (Ex: 480nm, Em: 520nm)
 
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.