Reaction Details | |||
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Cell Reactant: | Streptavadin(N23E) | ||
Syringe Reactant: | BDBM12 | ||
Meas. Tech.: | ITC | ||
Entry Date: | 12/19/00 | ||
Δ G ° : | n/a | ||
pH: | 7±n/a | ||
Log10Kb: | n/a | ||
Temperature: | 298.15±n/a (K) | ||
ΔH° : | -24.0± (kcal/mole) | ||
ΔHobs : | -24.0± (kcal/mole) | ||
Ionic Strength: | n/a | ||
n/a | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | n/a | ||
Comments: | n/a | ||
Citation | Klumb, LA; Chu, V; Stayton, PS Energetic roles of hydrogen bonds at the ureido oxygen binding pocket in the streptavidin-biotin complex. Biochemistry37:7657-63 (1998) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
Streptavadin(N23E) | |||
Source: | Protein was expressed in a T7 expression system (pET-21a, Novagen Inc.,Madison,WI) as insoluble inclusion bodies. | ||
Purity: | n/a | ||
Prep. Method: | Refolded protein was purified by affinity chromatography with iminobiotin-agarose. | ||
Name: | Streptavadin(N23E) | ||
Synonyms: | streptavidin (N23E) | ||
Type: | Protein | ||
Mol. Mass.: | 16504.73 | ||
Organism: | Streptomyces avidinii | ||
Description: | Biotin-Binding Protein | ||
Residue: | 159 | ||
Sequence: |
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BDBM12 | |||
Source: | n/a | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM12 | ||
Synonyms: | 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin | cid_171548 | cid_253 | ||
Type | Enzyme co-factor | ||
Emp. Form. | C10H16N2O3S | ||
Mol. Mass. | 244.311 | ||
SMILES | [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2 |r| | ||
Structure |
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