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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Streptavadin(N23A) | ||
| Syringe Reactant: | BDBM12 | ||
| Meas. Tech.: | ITC | ||
| Entry Date: | 12/19/00 | ||
| Δ G ° : | n/a | ||
| pH: | 7±n/a | ||
| Log10Kb: | n/a | ||
| Temperature: | 298.15±n/a (K) | ||
| ΔH° : | -18.0± (kcal/mole) | ||
| ΔHobs : | -18.0± (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| n/a | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | n/a | ||
| ΔS° : | n/a | ||
| Comments: | n/a | ||
| Citation |  Klumb, LA; Chu, V; Stayton, PS Energetic roles of hydrogen bonds at the ureido oxygen binding pocket in the streptavidin-biotin complex. Biochemistry37:7657-63 (1998) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
| Streptavadin(N23A) | |||
| Source: | Protein was expressed in a T7 expression system (pET-21a, Novagen Inc.,Madison,WI) as insoluble inclusion bodies. | ||
| Purity: | n/a | ||
| Prep. Method: | Refolded protein was purified by affinity chromatography with iminobiotin-agarose. | ||
| Name: | Streptavadin(N23A) | ||
| Synonyms: | streptavadin (N23A) | ||
| Type: | Protein | ||
| Mol. Mass.: | 16447.70 | ||
| Organism: | Streptomyces avidinii | ||
| Description: | Biotin-Binding Protein | ||
| Residue: | 159 | ||
| Sequence: |
| ||
| BDBM12 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name | BDBM12 | ||
| Synonyms: | 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin | cid_171548 | cid_253 | ||
| Type | Enzyme co-factor | ||
| Emp. Form. | C10H16N2O3S | ||
| Mol. Mass. | 244.311 | ||
| SMILES | [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2 |r| | ||
| Structure | 
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