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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | CAM-RNase A | ||
| Syringe Reactant: | SecB | ||
| Meas. Tech.: | ITC | ||
| Entry Date: | 12/13/00 | ||
| ΔG°: | -6.63± (kcal/mole) | ||
| pH: | 7.4±n/a | ||
| Log10Kb: | 4.90± n/a | ||
| Temperature: | 293.15±n/a (K) | ||
| ΔH° : | -11.9± (kcal/mole) | ||
| ΔHobs: | n/a | ||
| Ionic Strength: | n/a | ||
| n/a | |||
| Protons Released: | n/a | ||
| ΔCp : | -0.41±n/a (kcal/mole) | ||
| Stoich. Param.: | 1 | ||
| ΔS° : | -0.0200± (kcal/mole-K) | ||
| Comments: | discrepancies in the fluorescence and calorimetric-derived binding constants | ||
| Citation |  Panse, VG; Swaminathan, CP; Surolia, A; Varadarajan, R Thermodynamics of substrate binding to the chaperone SecB. Biochemistry39:2420-7 (2000) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
| CAM-RNase A | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name: | Ribonuclease pancreatic | ||
| Synonyms: | CAM-RNase A | RNAS1_BOVIN | RNASE1 | RNAse A | RNS1 | ||
| Type: | Enzyme | ||
| Mol. Mass.: | 16468.79 | ||
| Organism: | Bison bison (American bison) | ||
| Description: | carboxamidomethylated RNase A | ||
| Residue: | 150 | ||
| Sequence: |
| ||
| SecB | |||
| Source: | Expressed in E. coli strain BL21 (DE3) | ||
| Purity: | 99% | ||
| Prep. Method: | Fast flow Q-Sepharose column, and then a Sephacryl S-200 gel filtration column | ||
| Name: | SecB | ||
| Synonyms: | n/a | ||
| Type: | Protein | ||
| Mol. Mass.: | 17264.58 | ||
| Organism: | Escherichia coli | ||
| Description: | D6JGS5 | ||
| Residue: | 155 | ||
| Sequence: |
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