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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | HyHEL-5 Mutant(E50D) | ||
| Syringe Reactant: | Lysozyme (hen egg) | ||
| Meas. Tech.: | ITC and sedimentation equilibrium | ||
| Entry Date: | 12/13/00 | ||
| ΔG°: | -10.6±0.100 (kcal/mole) | ||
| pH: | 8±n/a | ||
| Log10Kb: | 7.75± n/a | ||
| Temperature: | 298.15±n/a (K) | ||
| ΔH° : | -18.8±0.600 (kcal/mole) | ||
| ΔHobs : | -18.8± (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| not known | |||
| Protons Released: | n/a | ||
| ΔCp : | -0.27±0.04 (kcal/mole) | ||
| Stoich. Param.: | 1 | ||
| ΔS° : | -0.0300±0 (kcal/mole-K) | ||
| Comments: | n/a | ||
| Citation |  Wibbenmeyer, JA; Schuck, P; Smith-Gill, SJ; Willson, RC Salt links dominate affinity of antibody HyHEL-5 for lysozyme through enthalpic contributions. J Biol Chem274:26838-42 (1999) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| HyHEL-5 Mutant(E50D) | |||
| Source: | Site-directed mutagenesis of recombinant HyHEL-5 Fab | ||
| Purity: | 95% | ||
| Prep. Method: | Refolded protein was purified by Q Sepharose and nickel-charged chelating Sepharose Fast Flow Chromatography | ||
| Name: | HyHEL-5 Mutant(E50D) | ||
| Synonyms: | HyHEL-5 Fab mutant (E50D) | ||
| Type: | Antibody | ||
| Mol. Mass.: | 22997.82 | ||
| Organism: | Mus musculus (mouse) | ||
| Description: | mutant of murine monoclonal antibody HyHEL-5 to hen egg lysozyme | ||
| Residue: | 215 | ||
| Sequence: |
| ||
| Lysozyme (hen egg) | |||
| Source: | Worthington Biologicals | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name: | Lysozyme C | ||
| Synonyms: | FvD1.3(VLW92A) | FvD1.3(VLW92D) | FvD1.3(VLW92F) | FvD1.3(VLW92H) | FvD1.3(VLW92S) | FvD1.3(VLW92V) | FvD1.3(VLW92wt) | LYSC_CHICK | LYZ | Lysozyme (hen egg) | hen egg lysozyme (HEL) | ||
| Type: | Enzyme | ||
| Mol. Mass.: | 16248.07 | ||
| Organism: | Gallus gallus (chicken) | ||
| Description: | P00698 | ||
| Residue: | 147 | ||
| Sequence: |
| ||
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