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Cell Reactant:Acetate Operon Repressor (IclR)
Syringe Reactant:BDBM19473
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:12/10/07
 
Δ G ° :n/a
pH:7.4±n/a
Log10Kb:3.49± 2.60
Temperature:298.15±n/a (K)
ΔH° :n/a
ΔHobs :-5.80± (kcal/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:no
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:n/a
ΔS° :n/a
Comments:n/a
 
Citation Lorca, GLEzersky, ALunin, VVWalker, JRAltamentova, SEvdokimova, EVedadi, MBochkarev, ASavchenko, A Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem282:16476-91 (2007) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
Acetate Operon Repressor (IclR)
Source:His-tagged fusion protein was overexpressed and purified in E. coli.
Purity:n/a
Prep. Method:The His6 tag was cleaved from the protein by tobacco etch virus protease. The cleaved protein was then resolved by flowing the mixture through a second Ni2+ column. The purified proteins were dialyzed and concentrated.
Name:Acetate Operon Repressor (IclR)
Synonyms:ICLR_ECOLI | iclR
Type:Transcriptional regulator
Mol. Mass.:29746.52
Organism:Escherichia coli
Description:n/a
Residue:274
Sequence:
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
HRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETV
NMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHR
KGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM19473
Source:n/a
Purity:n/a
Prep. Method:n/a
NameBDBM19473
Synonyms:2-Oxopropanoate | 2-oxopropanoic acid | Pyruvate
TypeSmall organic molecule
Emp. Form.C3H4O3
Mol. Mass.88.0621
SMILESCC(=O)C(O)=O
Structure
Search PDB for entries with ligand similarity:Similarity to this molecule at least:

   
    

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Last update November 1, 2007
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