Reaction Details | |||
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Cell Reactant: | Acetate Operon Repressor (IclR) | ||
Syringe Reactant: | BDBM19473 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 12/10/07 | ||
Δ G ° : | n/a | ||
pH: | 7.4±n/a | ||
Log10Kb: | 3.49± 2.60 | ||
Temperature: | 298.15±n/a (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -5.80± (kcal/mole) | ||
Ionic Strength: | n/a | ||
no | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | n/a | ||
Comments: | n/a | ||
Citation | Lorca, GL; Ezersky, A; Lunin, VV; Walker, JR; Altamentova, S; Evdokimova, E; Vedadi, M; Bochkarev, A; Savchenko, A Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem282:16476-91 (2007) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
Acetate Operon Repressor (IclR) | |||
Source: | His-tagged fusion protein was overexpressed and purified in E. coli. | ||
Purity: | n/a | ||
Prep. Method: | The His6 tag was cleaved from the protein by tobacco etch virus protease. The cleaved protein was then resolved by flowing the mixture through a second Ni2+ column. The purified proteins were dialyzed and concentrated. | ||
Name: | Acetate Operon Repressor (IclR) | ||
Synonyms: | ICLR_ECOLI | iclR | ||
Type: | Transcriptional regulator | ||
Mol. Mass.: | 29746.52 | ||
Organism: | Escherichia coli | ||
Description: | n/a | ||
Residue: | 274 | ||
Sequence: |
| ||
BDBM19473 | |||
Source: | n/a | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM19473 | ||
Synonyms: | 2-Oxopropanoate | 2-oxopropanoic acid | Pyruvate | ||
Type | Small organic molecule | ||
Emp. Form. | C3H4O3 | ||
Mol. Mass. | 88.0621 | ||
SMILES | CC(=O)C(O)=O | ||
Structure |
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