Reaction Details | |||
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Cell Reactant: | Cytochrome P450 2B4 Mutant (F206A) | ||
Syringe Reactant: | BDBM19475 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 12/10/07 | ||
Δ G ° : | n/a | ||
pH: | 7.4±n/a | ||
Log10Kb: | 6.33± n/a | ||
Temperature: | 298.15±n/a (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -45.6± (kJ/mole) | ||
Ionic Strength: | n/a | ||
no | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | n/a | ||
Comments: | n/a | ||
Citation | Wang, BZ; Wang, J; Wu, Q [BRCA1 protein expression in sporadic breast and its clinical significance]. Zhonghua Wai Ke Za Zhi41:186-8 (2003) [PubMed] | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
Cytochrome P450 2B4 Mutant (F206A) | |||
Source: | Purified from E. coli. | ||
Purity: | n/a | ||
Prep. Method: | Cold ethanol was added to the protein to a final concentration of 2% just prior to the temperature equilibration, and the sample was loaded into the calorimetric cell. | ||
Name: | Cytochrome P450 2B4 Mutant (F206A) | ||
Synonyms: | 2B4dH(H226Y) mutant F206A | CYPIIB4 | N-terminal truncated and modified form of P450 2B4 | P450 types B0 and B1 | P450-LM2 | ||
Type: | Enzyme | ||
Mol. Mass.: | 54034.11 | ||
Organism: | Oryctolagus cuniculus (rabbit) | ||
Description: | F206A was created using 2B4dH(H226Y) as a template. 2B4dH(H226Y) is an N-terminal truncated and modified and C-terminal His-tagged form of P450 2B4 engineered for enhanced solubility and containing a His- 226 to Tyr substitution to prevent dimerization. | ||
Residue: | 476 | ||
Sequence: |
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BDBM19475 | |||
Source: | Sigma-Aldrich | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM19475 | ||
Synonyms: | 4-(4-chlorophenyl)imidazole | 4-CPI | 5-(4-chlorophenyl)-1H-imidazole | ||
Type | Small organic molecule | ||
Emp. Form. | C9H7ClN2 | ||
Mol. Mass. | 178.618 | ||
SMILES | Clc1ccc(cc1)-c1c[nH]cn1 | ||
Structure |
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