Reaction Details | |||
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Cell Reactant: | BirA Bifunctional Protein | ||
Syringe Reactant: | BDBM19478 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 12/09/07 | ||
ΔG°: | -13.9±0.400 (kcal/mole) | ||
pH: | 7.5±0.02 | ||
Log10Kb: | 10.4± 10.1 | ||
Temperature: | 293.15±0.1 (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -12.3±0.900 (kcal/mole) | ||
Ionic Strength: | n/a | ||
no | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | 0.91 | ||
ΔS° : | 0.0100±0 (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Naganathan, S; Beckett, D Nucleation of an allosteric response via ligand-induced loop folding. J Mol Biol373:96-111 (2007) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
BirA Bifunctional Protein | |||
Source: | The BirA variants were expressed in the Escherichia coli strain JM109 transformed with the plasmid pBtac2 carrying the E. coli birA gene (wild-type or mutant) under transcriptional control of the tac. | ||
Purity: | >95% | ||
Prep. Method: | The separation and purification of BirA from other cellular proteins was carried out in NiNTA column and Sepharose chromatography steps. | ||
Name: | Bifunctional ligase/repressor BirA | ||
Synonyms: | BIRA_ECOLI | Biotin operon repressor | Biotin protein ligase (BPL) | Biotin--[acetyl-CoA-carboxylase] synthetase | BirA Bifunctional Protein | bioR | birA | dhbB | ||
Type: | Repressor protein | ||
Mol. Mass.: | 35313.58 | ||
Organism: | Escherichia coli | ||
Description: | n/a | ||
Residue: | 321 | ||
Sequence: |
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BDBM19478 | |||
Source: | Synthesized and purified in the laboratory | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM19478 | ||
Synonyms: | BTX | bio-5-AMP | biotinyl-5-AMP | {[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}({5-[(4S)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoyl}oxy)phosphinic acid | ||
Type | Nucleoside or nucleotide | ||
Emp. Form. | C20H28N7O9PS | ||
Mol. Mass. | 573.517 | ||
SMILES | Nc1ncnc2n(cnc12)[C@@H]1O[C@H](COP(O)(=O)OC(=O)CCCC[C@@H]2SCC3NC(=O)NC23)[C@@H](O)[C@H]1O |r| | ||
Structure |
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