Reaction Details | |||
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Cell Reactant: | BirA Bifunctional Protein | ||
Syringe Reactant: | BDBM12 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 12/19/07 | ||
ΔG°: | -41.3±0.167 (kJ/mole) | ||
pH: | 7.5±0.2 | ||
Log10Kb: | 7.38± 6.23 | ||
Temperature: | 293.15±0.1 (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -80.3±0.418 (kJ/mole) | ||
Ionic Strength: | n/a | ||
no | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | -0.1326±0.00140 (kJ/mole-K) | ||
Comments: | n/a | ||
Citation | Brown, PH; Beckett, D Use of binding enthalpy to drive an allosteric transition. Biochemistry44:3112-21 (2005) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
BirA Bifunctional Protein | |||
Source: | The BirA variants were expressed in the Escherichia coli strain JM109 transformed with the plasmid pBtac2 carrying the E. coli birA gene (wild-type or mutant) under transcriptional control of the tac | ||
Purity: | >95% | ||
Prep. Method: | The separation and purification of BirA from other cellular proteins was carried out in NiNTA column and Sepharose chromatography steps. | ||
Name: | Bifunctional ligase/repressor BirA | ||
Synonyms: | BIRA_ECOLI | Biotin operon repressor | Biotin protein ligase (BPL) | Biotin--[acetyl-CoA-carboxylase] synthetase | BirA Bifunctional Protein | bioR | birA | dhbB | ||
Type: | Repressor protein | ||
Mol. Mass.: | 35313.58 | ||
Organism: | Escherichia coli | ||
Description: | n/a | ||
Residue: | 321 | ||
Sequence: |
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BDBM12 | |||
Source: | Sigma-Aldrich | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM12 | ||
Synonyms: | 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin | cid_171548 | cid_253 | ||
Type | Enzyme co-factor | ||
Emp. Form. | C10H16N2O3S | ||
Mol. Mass. | 244.311 | ||
SMILES | [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2 |r| | ||
Structure |
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