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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | BirA Bifunctional Protein | ||
| Syringe Reactant: | BDBM12 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 12/19/07 | ||
| ΔG°: | -9.89±0.0400 (kcal/mole) | ||
| pH: | 7.5±0.2 | ||
| Log10Kb: | 7.38± 6.23 | ||
| Temperature: | 293.15±0.1 (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -19.2±0.100 (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | n/a | ||
| ΔS° : | -0.0300±0 (kcal/mole-K) | ||
| Comments: | n/a | ||
| Citation |  Brown, PH; Beckett, D Use of binding enthalpy to drive an allosteric transition. Biochemistry44:3112-21 (2005) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| BirA Bifunctional Protein | |||
| Source: | The BirA variants were expressed in the Escherichia coli strain JM109 transformed with the plasmid pBtac2 carrying the E. coli birA gene (wild-type or mutant) under transcriptional control of the tac | ||
| Purity: | >95% | ||
| Prep. Method: | The separation and purification of BirA from other cellular proteins was carried out in NiNTA column and Sepharose chromatography steps. | ||
| Name: | Bifunctional ligase/repressor BirA | ||
| Synonyms: | BIRA_ECOLI | Biotin operon repressor | Biotin protein ligase (BPL) | Biotin--[acetyl-CoA-carboxylase] synthetase | BirA Bifunctional Protein | bioR | birA | dhbB | ||
| Type: | Repressor protein | ||
| Mol. Mass.: | 35313.58 | ||
| Organism: | Escherichia coli | ||
| Description: | n/a | ||
| Residue: | 321 | ||
| Sequence: |
| ||
| BDBM12 | |||
| Source: | Sigma-Aldrich | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name | BDBM12 | ||
| Synonyms: | 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin | cid_171548 | cid_253 | ||
| Type | Enzyme co-factor | ||
| Emp. Form. | C10H16N2O3S | ||
| Mol. Mass. | 244.311 | ||
| SMILES | [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2 |r| | ||
| Structure | 
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