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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Streptavadin(W120F) | ||
| Syringe Reactant: | BDBM12 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 04/30/08 | ||
| Δ G ° : | n/a | ||
| pH: | n/a | ||
| Log10Kb: | n/a | ||
| Temperature: | 298.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -19.4± (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | 0.9 | ||
| ΔS° : | n/a | ||
| Comments: | n/a | ||
| Citation |  Chilkoti, A; Boyer, FE; Stayton, PS; Vara Prasad, JV; Domagala, JM; Ellsworth, EL; Gajda, C; Hagen, SE; Markoski, LJ; Tait, BD; Lunney, EA; Palovsky, A; Ferguson, D; Graham, N; Holler, T; Hupe, D; Nouhan, C; Tummino, PJ; Urumov, A; Zeikus, E; Zeikus, G; Gracheck, SJ; Sanders, JM; VanderRoest, S; Brodfuehrer, J; Iyer, K; Sinz, M; Gulnik, SV Molecular Origins of the Slow Streptavidin-Biotin Dissociation Kinetics J Am Chem Soc117:10622-10628 (1995) Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Streptavadin(W120F) | |||
| Source: | Mutant protein was expressed in a T7 expression system (pET-21a, Novagen Inc.,Madison,WI) as insoluble inclusion bodies. | ||
| Purity: | n/a | ||
| Prep. Method: | Refolded protein was purified by affinity chromatography with iminobiotin-agarose. | ||
| Name: | Streptavadin(W120F) | ||
| Synonyms: | n/a | ||
| Type: | Biotin-Binding Protein | ||
| Mol. Mass.: | 18796.50 | ||
| Organism: | Streptomyces avidinii | ||
| Description: | n/a | ||
| Residue: | 183 | ||
| Sequence: |
| ||
| BDBM12 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name | BDBM12 | ||
| Synonyms: | 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin | cid_171548 | cid_253 | ||
| Type | Enzyme co-factor | ||
| Emp. Form. | C10H16N2O3S | ||
| Mol. Mass. | 244.311 | ||
| SMILES | [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2 |r| | ||
| Structure | 
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