Reaction Details | |||
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Cell Reactant: | H1/H5-depleted chromatin | ||
Syringe Reactant: | BDBM18 | ||
Meas. Tech.: | ITC | ||
Entry Date: | 12/11/00 | ||
ΔG°: | -6.63±0.0300 (kcal/mole) | ||
pH: | 7.5±n/a | ||
Log10Kb: | 4.93± 3.54 | ||
Temperature: | 293.15±n/a (K) | ||
ΔH° : | -8.14± (kcal/mole) | ||
ΔHobs : | -8.14± (kcal/mole) | ||
Ionic Strength: | n/a | ||
n/a | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | -0.0100±0 (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Taquet, A; Labarbe, R; Houssier, C Calorimetric investigation of ethidium and netropsin binding to chicken erythrocyte chromatin. Biochemistry37:9119-26 (1998) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
H1/H5-depleted chromatin | |||
Source: | Obtained from freshly killed chicken | ||
Purity: | n/a | ||
Prep. Method: | nuclei were lysed and dialyzed against buffer, soluble chromatin were collected by centrifugation.H1 and H5 histones were depleted by adding NaCl 4M to chromatin solution up to 650 mM | ||
Name: | H1/H5-depleted chromatin | ||
Synonyms: | n/a | ||
Type: | Other | ||
Mol. Mass.: | n/a | ||
Description: | H1/H5 were depleted from chicken erythrocyte chromatin | ||
Components: | This complex has 0 components. | ||
Component 1 | |||
Name: | Histone deacetylase 1 | ||
Synonyms: | Cereblon/Histone deacetylase 1 | HD1 | HDAC1 | HDAC1_HUMAN | Histone deacetylase 1 (HDAC1) | Human HDAC1 | RPD3L1 | ||
Type: | Enzyme | ||
Mol. Mass.: | 55090.27 | ||
Organism: | Homo sapiens (Human) | ||
Description: | Q13547 | ||
Residue: | 482 | ||
Sequence: |
| ||
Component 2 | |||
Name: | Histone deacetylase 4 | ||
Synonyms: | Cereblon/Histone deacetylase 4 | HD4 | HDAC4 | HDAC4_HUMAN | Histone acetylase 4(HDAC4) | Human HDAC4 | KIAA0288 | ||
Type: | Enzyme | ||
Mol. Mass.: | 119049.39 | ||
Organism: | Homo sapiens (Human) | ||
Description: | P56524 | ||
Residue: | 1084 | ||
Sequence: |
| ||
BDBM18 | |||
Source: | Sigma Chemical Co. | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM18 | ||
Synonyms: | (3-amino-5-methyl-6-phenyl-phenanthridin-5-ium-8-yl)amine;bromide | 3,8-diamino-5-methyl-6-phenylphenanthridin-5-ium bromide | 5-methyl-6-phenyl-phenanthridin-5-ium-3,8-diamine;bromide | 5-methyl-6-phenylphenanthridin-5-ium-3,8-diamine;bromide | Dimidium bromide | MLS001074889 | SMR000568409 | cid_68207 | ethidium bromide | ||
Type | Small organic molecule | ||
Emp. Form. | C20H18N3 | ||
Mol. Mass. | 300.3765 | ||
SMILES | Cn1c(-c2ccccc2)c2cc(N)ccc2c2ccc(=[NH2+])cc12 | ||
Structure |
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