Reaction Details | |||
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Cell Reactant: | Rho Transcription Termination Factor | ||
Syringe Reactant: | BDBM22601 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 06/14/08 | ||
ΔG°: | -29.8±0.0836 (kJ/mole) | ||
pH: | 7.9±n/a | ||
Log10Kb: | 5.20± 3.70 | ||
Temperature: | 299.15±n/a (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -5.60±2.59 (kJ/mole) | ||
Ionic Strength: | n/a | ||
no | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | 6 | ||
ΔS° : | 0.0808±0.00360 (kJ/mole-K) | ||
Comments: | The stoichiometry parameter was fixed to six, or for the two site model, two integers with a sum of six. | ||
Citation | Brogan, AP; Widger, WR; Bensadek, D; Riba-Garcia, I; Gaskell, SJ; Kohn, H Development of a technique to determine bicyclomycin-rho binding and stoichiometry by isothermal titration calorimetry and mass spectrometry. J Am Chem Soc127:2741-51 (2005) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
Rho Transcription Termination Factor | |||
Source: | The open reading frame encoding the full-length Escherichia coli Rho protein was PCR amplified and cloned. The protein was overexpressed in E. coli strain BL21 (pLysS). | ||
Purity: | 99% | ||
Prep. Method: | The protein was twice purified over a POROS-HS column (Perseptive Biosystems) then further purified with two successive Sephacryl S-300 (Amersham) sizing columns. The protein at this stage was more than 99% pure as judged by SDS-PAGE. | ||
Name: | Rho Transcription Termination Factor | ||
Synonyms: | ATP-dependent helicase rho | RHO_ECOLI | nitA | psuA | rho | rnsC | sbaA | tsu | ||
Type: | Enzyme | ||
Mol. Mass.: | 47006.70 | ||
Organism: | Escherichia coli (strain K12) | ||
Description: | Rho is a hexameric RNA/DNA helicase/translocase. | ||
Residue: | 419 | ||
Sequence: |
| ||
BDBM22601 | |||
Source: | n/a | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name | BDBM22601 | ||
Synonyms: | 3-({[(1S,6R)-6-hydroxy-8,10-dioxo-1-[(1S,2S)-1,2,3-trihydroxy-2-methylpropyl]-2-oxa-7,9-diazabicyclo[4.2.2]decan-5-yl]methyl}sulfanyl)benzaldehyde | 5a-(3-formylphenylsulfanyl)-dihydrobicyclomycin | FPD | FPDB | ||
Type | Antibiotic | ||
Emp. Form. | C19H24N2O8S | ||
Mol. Mass. | 440.467 | ||
SMILES | C[C@](O)(CO)[C@H](O)[C@@]12NC(=O)[C@@](O)(NC1=O)C(CSc1cccc(C=O)c1)CCO2 |TLB:10:9:13.14:29.16.28.27,THB:15:14:8.9:29.16.28.27,17:16:13.14:8.9| | ||
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