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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Major Urinary Protein (MUP-1) | ||
| Syringe Reactant: | BDBM22606 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 06/15/08 | ||
| ΔG°: | -35.6±0.100 (kJ/mole) | ||
| pH: | 7.4±n/a | ||
| Log10Kb: | 6.20± 5.18 | ||
| Temperature: | 300±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -58±0.600 (kJ/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | 1.05 | ||
| ΔS° : | -0.0747±0.00200 (kJ/mole-K) | ||
| Comments: | Kd value was obtained from J. Mol. Biol. (2006) 362,994-1003. | ||
| Citation |  Malham, R; Johnstone, S; Bingham, RJ; Barratt, E; Phillips, SE; Laughton, CA; Homans, SW Strong solute-solute dispersive interactions in a protein-ligand complex. J Am Chem Soc127:17061-7 (2005) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Major Urinary Protein (MUP-1) | |||
| Source: | The MUP-1 gene was cloned and expressed in E. coli cells. | ||
| Purity: | n/a | ||
| Prep. Method: | MUP-I was purified using Ni-NTA resin. To remove endogenous bound ligands, an ethanol precipitation step was utilized involving addition of 2 volumes of absolute ethanol per volume of protein solution, followed by incubation at 4 °C for 2 h. After centrifugation at 5000g, the pellet was lyophilised, resuspended in PBS pH 7.4, and dialyzed overnight against the same buffer. | ||
| Name: | Major Urinary Protein (MUP-1) | ||
| Synonyms: | MUP1_MOUSE | Major urinary protein 1 precursor | Mup1 | ||
| Type: | Other Protein Type | ||
| Mol. Mass.: | 20640.17 | ||
| Organism: | Mus musculus (mouse) | ||
| Description: | n/a | ||
| Residue: | 180 | ||
| Sequence: |
| ||
| BDBM22606 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name | BDBM22606 | ||
| Synonyms: | 1-octanol | Octan-1-ol | n-Octyl Alcohol | ||
| Type | Small organic molecule | ||
| Emp. Form. | C8H18O | ||
| Mol. Mass. | 130.2279 | ||
| SMILES | CCCCCCCCO | ||
| Structure | 
| ||
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