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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | apocytochrome b562 | ||
| Syringe Reactant: | BDBM20 | ||
| Meas. Tech.: | ITC | ||
| Entry Date: | 12/04/00 | ||
| ΔG°: | -11.1±0.200 (kcal/mole) | ||
| pH: | 8.5±n/a | ||
| Log10Kb: | 8.04± 8 | ||
| Temperature: | 298.15±n/a (K) | ||
| ΔH° : | -17.1± (kcal/mole) | ||
| ΔHobs : | -17.1± (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| n/a | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | n/a | ||
| ΔS° : | -0.0200±0.0100 (kcal/mole-K) | ||
| Comments: | binding is very tight, limiting the accuracy of calorimetric measurement of the binding constant to approximately 25% | ||
| Citation |  Robinson, CR; Liu, Y; Thomson, JA; Sturtevant, JM; Sligar, SG Energetics of heme binding to native and denatured states of cytochrome b562. Biochemistry36:16141-6 (1997) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
| apocytochrome b562 | |||
| Source: | E.coli | ||
| Purity: | n/a | ||
| Prep. Method: | Holoprotein was purified by ion-exchange FPLC just prior to heme extraction | ||
| Name: | apocytochrome b562 | ||
| Synonyms: | C562_ECOLX | apocytochrome b562(denatured) | cybC | denatured apocytochrome b562 | ||
| Type: | Protein | ||
| Mol. Mass.: | 14060.82 | ||
| Organism: | Escherichia coli | ||
| Description: | P0ABE7 | ||
| Residue: | 128 | ||
| Sequence: |
| ||
| BDBM20 | |||
| Source: | Sigma Chemical Co. | ||
| Purity: | n/a | ||
| Prep. Method: | dissolved in NaOH and diluted with buffer | ||
| Name | BDBM20 | ||
| Synonyms: | 3-[5-(2-carboxyethyl)-15,20-diethenyl-22-hydroxy-4,10,14,19,24-pentamethyl-21,23,24,25-tetraaza-22-ferrahexacyclo[9.9.3.1^{3,6}.1^{13,16}.0^{8,23}.0^{18,21}]pentacosa-1(20),3,5,8,10,13,15,18-octaen-9-yl]propanoic acid | Hematin(iron protoporphyrin IX) | ||
| Type | Porphyrin | ||
| Emp. Form. | C35H40N4O4 | ||
| Mol. Mass. | 580.7176 | ||
| SMILES | Cc1c2Cc3[n-]c(Cc4c(C=C)c(C)c(Cc5[n-]c(Cc([nH]2)c1CCC(O)=O)c(CCC(O)=O)c5C)n4C)c(C)c3C=C | ||
| Structure | 
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