Search and Browse
Download
Enter Data
| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Transcriptional Regulator TtgR | ||
| Syringe Reactant: | BDBM23451 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 07/23/08 | ||
| ΔG°: | -27.5±0.334 (kJ/mole) | ||
| pH: | 7±n/a | ||
| Log10Kb: | 4.74± 3.84 | ||
| Temperature: | 303.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -7.02±1.04 (kJ/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | n/a | ||
| ΔS° : | 0.0674±0.00370 (kJ/mole-K) | ||
| Comments: | n/a | ||
| Citation |  Terán, W; Krell, T; Ramos, JL; Gallegos, MT Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem281:7102-9 (2006) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Transcriptional Regulator TtgR | |||
| Source: | A 651-bp fragment containing the ttgR gene was amplified by PCR from P. putida DOT-T1E chromosomal DNA, and cloned in E. coli expression vector. TtgR protein was over-expressed and purified. | ||
| Purity: | n/a | ||
| Prep. Method: | TtgR protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer. | ||
| Name: | Transcriptional Regulator TtgR | ||
| Synonyms: | HTH-type transcriptional regulator ttgR | TTGR_PSEPT | Toluene efflux pump ttgABC operon repressor | ttgR | ||
| Type: | Repressor; homodimer | ||
| Mol. Mass.: | 23852.57 | ||
| Organism: | Pseudomonas putida | ||
| Description: | n/a | ||
| Residue: | 210 | ||
| Sequence: |
| ||
| BDBM23451 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | All chemicals were manipulated in glass vessels and effector samples were neither degassed nor filtered, to avoid evaporation or nonspecific binding. Analyses were carried out using 5% DMSO to facilitate solubilization. | ||
| Name | BDBM23451 | ||
| Synonyms: | 5,14-dihydroxy-8,17-dioxatetracyclo[8.7.0.0^{2,7}.0^{11,16}]heptadeca-1(10),2,4,6,11(16),12,14-heptaen-9-one | 7,12-Dihydroxycoumestan | CHEMBL30707 | Chrysanthin | Coumestrol | Cumoestrol | US8552057, 3 | ||
| Type | Coumestan | ||
| Emp. Form. | C15H8O5 | ||
| Mol. Mass. | 268.221 | ||
| SMILES | Oc1ccc2c(c1)oc1c2c(=O)oc2cc(O)ccc12 | ||
| Structure | 
| ||
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||