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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Transcriptional Regulator TtgR-DNA | ||
| Syringe Reactant: | BDBM7460 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 07/23/08 | ||
| ΔG°: | -7.95±0.0900 (kcal/mole) | ||
| pH: | 7±n/a | ||
| Log10Kb: | 5.73± 4.89 | ||
| Temperature: | 303.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -5.13±0.210 (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | n/a | ||
| ΔS° : | 0.0100±0 (kcal/mole-K) | ||
| Comments: | n/a | ||
| Citation |  Terán, W; Krell, T; Ramos, JL; Gallegos, MT Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem281:7102-9 (2006) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Transcriptional Regulator TtgR-DNA | |||
| Source: | TtgR protein was over-expressed and purified from E. coli. DNA fragment was obtained by annealing two complementary oligonucleotides. | ||
| Purity: | n/a | ||
| Prep. Method: | Purified TtgR was placed into the ITC cell and titrated by the addition of the 40-bp DNA fragment. The completeness of protein saturation with DNA was verified by polyacrylamide gel electrophoresis. | ||
| Name: | Transcriptional Regulator TtgR-DNA | ||
| Synonyms: | n/a | ||
| Type: | DNA Complex | ||
| Mol. Mass.: | n/a | ||
| Description: | n/a | ||
| Components: | This complex has 2 components. | ||
| Component 1 | |||
| Name: | Transcriptional Regulator TtgR | ||
| Synonyms: | HTH-type transcriptional regulator ttgR | TTGR_PSEPT | Toluene efflux pump ttgABC operon repressor | ttgR | ||
| Type: | Repressor; homodimer | ||
| Mol. Mass.: | 23852.57 | ||
| Organism: | Pseudomonas putida | ||
| Description: | n/a | ||
| Residue: | 210 | ||
| Sequence: |
| ||
| Component 2 | |||
| Name: | TtgR Operator-Containing DNA Fragment | ||
| Synonyms: | n/a | ||
| Type: | Duplex DNA | ||
| Mol. Mass.: | 3377.80 | ||
| Organism: | n/a | ||
| Description: | n/a | ||
| Residue: | 40 | ||
| Sequence: |
| ||
| BDBM7460 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | All chemicals were manipulated in glass vessels and effector samples were neither degassed nor filtered, to avoid evaporation or nonspecific binding. | ||
| Name | BDBM7460 | ||
| Synonyms: | 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one | 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-chromen-4-one | 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-chromone;hydrate | CHEMBL50 | Quercetin | Quercetin (10) | Quercetin (21) | Quercetin (Qur) | US11021454, Compound Quercetin | US9180183, Quercetin | med.21724, Compound 4 | ||
| Type | Small organic molecule | ||
| Emp. Form. | C15H10O7 | ||
| Mol. Mass. | 302.2357 | ||
| SMILES | Oc1cc(O)c2c(c1)oc(-c1ccc(O)c(O)c1)c(O)c2=O | ||
| Structure | 
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