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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Transcriptional Regulator TtgR | ||
| Syringe Reactant: | BDBM7459 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 07/23/08 | ||
| ΔG°: | -7.13±0.0300 (kcal/mole) | ||
| pH: | 7±n/a | ||
| Log10Kb: | 5.14± 3.81 | ||
| Temperature: | 303.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -3.05±0.0900 (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | n/a | ||
| ΔS° : | 0.0100±0 (kcal/mole-K) | ||
| Comments: | n/a | ||
| Citation |  Terán, W; Krell, T; Ramos, JL; Gallegos, MT Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem281:7102-9 (2006) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Transcriptional Regulator TtgR | |||
| Source: | A 651-bp fragment containing the ttgR gene was amplified by PCR from P. putida DOT-T1E chromosomal DNA, and cloned in E. coli expression vector. TtgR protein was over-expressed and purified. | ||
| Purity: | n/a | ||
| Prep. Method: | TtgR protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer. | ||
| Name: | Transcriptional Regulator TtgR | ||
| Synonyms: | HTH-type transcriptional regulator ttgR | TTGR_PSEPT | Toluene efflux pump ttgABC operon repressor | ttgR | ||
| Type: | Repressor; homodimer | ||
| Mol. Mass.: | 23852.57 | ||
| Organism: | Pseudomonas putida | ||
| Description: | n/a | ||
| Residue: | 210 | ||
| Sequence: |
| ||
| BDBM7459 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | All chemicals were manipulated in glass vessels and effector samples were neither degassed nor filtered, to avoid evaporation or nonspecific binding. Analyses were carried out using 5% DMSO to facilitate solubilization. | ||
| Name | BDBM7459 | ||
| Synonyms: | 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one | 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-chromen-4-one | CHEMBL151 | Luteolin (27) | Luteolin (4) | acs.jmedchem.1c00409_ST.600 | cid_5280445 | luteolin | med.21724, Compound 3 | ||
| Type | Small organic molecule | ||
| Emp. Form. | C15H10O6 | ||
| Mol. Mass. | 286.2363 | ||
| SMILES | Oc1cc(O)c2c(c1)oc(cc2=O)-c1ccc(O)c(O)c1 | ||
| Structure | 
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