Reaction Details | |||
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Cell Reactant: | Transcriptional Regulator TtgR | ||
Syringe Reactant: | BDBM19459 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 07/22/08 | ||
ΔG°: | -6.33±0.0300 (kcal/mole) | ||
pH: | 7±n/a | ||
Log10Kb: | 4.56± 3.23 | ||
Temperature: | 303.15±n/a (K) | ||
ΔH° : | n/a | ||
ΔHobs : | -1.93±0.0600 (kcal/mole) | ||
Ionic Strength: | n/a | ||
no | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | 0.0100±0 (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Terán, W; Krell, T; Ramos, JL; Gallegos, MT Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem281:7102-9 (2006) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
Transcriptional Regulator TtgR | |||
Source: | A 651-bp fragment containing the ttgR gene was amplified by PCR from P. putida DOT-T1E chromosomal DNA, and cloned in E. coli expression vector. TtgR protein was over-expressed and purified. | ||
Purity: | n/a | ||
Prep. Method: | TtgR protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer. | ||
Name: | Transcriptional Regulator TtgR | ||
Synonyms: | HTH-type transcriptional regulator ttgR | TTGR_PSEPT | Toluene efflux pump ttgABC operon repressor | ttgR | ||
Type: | Repressor; homodimer | ||
Mol. Mass.: | 23852.57 | ||
Organism: | Pseudomonas putida | ||
Description: | n/a | ||
Residue: | 210 | ||
Sequence: |
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BDBM19459 | |||
Source: | n/a | ||
Purity: | n/a | ||
Prep. Method: | All chemicals were manipulated in glass vessels and effector samples were neither degassed nor filtered, to avoid evaporation or nonspecific binding. Analyses were carried out using 5% DMSO to facilitate solubilization. | ||
Name | BDBM19459 | ||
Synonyms: | 5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one | CHEMBL44 | Genistein | Genistein, 8 | Genisteol | Prunetol | US8552057, 2 | cid_5280961 | ||
Type | Phytoestrogen | ||
Emp. Form. | C15H10O5 | ||
Mol. Mass. | 270.2369 | ||
SMILES | Oc1ccc(cc1)-c1coc2cc(O)cc(O)c2c1=O | ||
Structure |
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