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Cell Reactant:Transcriptional Regulator TtgR
Syringe Reactant:BDBM23446
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:07/23/08
 
ΔG°:-42.6±0.418 (kJ/mole)
pH:7±n/a
Log10Kb:7.32± 6.60
Temperature:303.15±n/a (K)
ΔH° :n/a
ΔHobs :-63.1±0.418 (kJ/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:no
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:n/a
ΔS° : -0.0676±0.00280 (kJ/mole-K)
Comments:Thermodynamic parameters for the high affinity binding site; for the low affinity binding site, the K=460000 M-1, deltaG=-7.8 kcal/mol, deltaH=-1.8 kcal/mol.
 
Citation Alguel, YDe Lucca, GVLiang, JMeng, CTerán, WDe Lucca, IKrell, TRamos, JLGallegos, MTZhang, X Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials. J Mol Biol369:829-40 (2007) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
Transcriptional Regulator TtgR
Source:A 651-bp fragment containing the ttgR gene was amplified by PCR from P. putida DOT-T1E chromosomal DNA, and cloned in E. coli expression vector. TtgR protein was over-expressed and purified.
Purity:n/a
Prep. Method:TtgR protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer.
Name:Transcriptional Regulator TtgR
Synonyms:HTH-type transcriptional regulator ttgR | TTGR_PSEPT | Toluene efflux pump ttgABC operon repressor | ttgR
Type:Repressor; homodimer
Mol. Mass.:23852.57
Organism:Pseudomonas putida
Description:n/a
Residue:210
Sequence:
MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQ
ALLDSLHETHDHLARASESEDEVDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFT
DDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDAERAAVAMFAYVDGLIRRWLL
LPDSVDLLGDVEKWVDTGLDMLRLSPALRK
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM23446
Source:n/a
Purity:n/a
Prep. Method:n/a
NameBDBM23446
Synonyms:3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one | CHEMBL45068 | Phloretin | cid_4788
TypeDihydrochalcone
Emp. Form.C15H14O5
Mol. Mass.274.2687
SMILESOc1ccc(CCC(=O)c2c(O)cc(O)cc2O)cc1
Structure
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Last update November 1, 2007
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