Reaction Details | |||
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Cell Reactant: | HyHEL-10 Mutant (ETKF) | ||
Syringe Reactant: | Lysozyme (turkey egg) | ||
Meas. Tech.: | ITC | ||
Entry Date: | 12/20/00 | ||
ΔG°: | -11.8± (kcal/mole) | ||
pH: | 7.2±n/a | ||
Log10Kb: | 8.34± n/a | ||
Temperature: | 308±n/a (K) | ||
ΔH° : | -23.4± (kcal/mole) | ||
ΔHobs : | -23.4± (kcal/mole) | ||
Ionic Strength: | n/a | ||
n/a | |||
Protons Released: | n/a | ||
ΔCp : | -0.52±n/a (kcal/mole) | ||
Stoich. Param.: | n/a | ||
ΔS° : | -0.0400± (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Nishimiya, Y; Tsumoto, K; Shiroishi, M; Yutani, K; Kumagai, I Thermodynamic consequences of grafting enhanced affinity toward the mutated antigen onto an antibody. The case of anti-lysozyme antibody, HyHEL-10. J Biol Chem275:12813-20 (2000) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
HyHEL-10 Mutant (ETKF) | |||
Source: | E. coli strain BL21 (DE3) | ||
Purity: | n/a | ||
Prep. Method: | TEL-Sepharose column, and then Superdex 75 Prep Grade column | ||
Name: | HyHEL-10 Mutant (ETKF) | ||
Synonyms: | n/a | ||
Type: | Antibody | ||
Mol. Mass.: | 12789.90 | ||
Organism: | Mus musculus (mouse) | ||
Description: | HyHEL-10 mutations(Y53E,S54T,S56K,Y58F) in VH | ||
Residue: | 114 | ||
Sequence: |
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Lysozyme (turkey egg) | |||
Source: | Sigma | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name: | Lysozyme (turkey egg) | ||
Synonyms: | LYSC_MELGA | LYZ | turkey egg lysozyme (TEL) | ||
Type: | Enzyme | ||
Mol. Mass.: | 16145.52 | ||
Organism: | Meleagris gallopavo | ||
Description: | P00703 | ||
Residue: | 147 | ||
Sequence: |
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