Reaction Details | |||
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Cell Reactant: | HyHEL-10 Mutant (HY58F) | ||
Syringe Reactant: | Lysozyme (turkey egg) | ||
Meas. Tech.: | ITC | ||
Entry Date: | 12/20/00 | ||
ΔG°: | -11.9± (kcal/mole) | ||
pH: | 7.2±n/a | ||
Log10Kb: | 8.41± n/a | ||
Temperature: | 308±n/a (K) | ||
ΔH° : | -26.6± (kcal/mole) | ||
ΔHobs : | -26.6± (kcal/mole) | ||
Ionic Strength: | n/a | ||
n/a | |||
Protons Released: | n/a | ||
ΔCp : | -0.64±n/a (kcal/mole) | ||
Stoich. Param.: | n/a | ||
ΔS° : | -0.0500± (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Nishimiya, Y; Tsumoto, K; Shiroishi, M; Yutani, K; Kumagai, I Thermodynamic consequences of grafting enhanced affinity toward the mutated antigen onto an antibody. The case of anti-lysozyme antibody, HyHEL-10. J Biol Chem275:12813-20 (2000) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
HyHEL-10 Mutant (HY58F) | |||
Source: | Described in Rajpal, A., Taylor, M. G., and Kirsch, J. F. (1998) Protein Sci. 7, 1868-1874. | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name: | HyHEL-10 Mutant (HY58F) | ||
Synonyms: | n/a | ||
Type: | Antibody | ||
Mol. Mass.: | 12768.84 | ||
Organism: | Mus musculus (mouse) | ||
Description: | Mutation in heavy chain of HyHEL-10 | ||
Residue: | 114 | ||
Sequence: |
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Lysozyme (turkey egg) | |||
Source: | Sigma | ||
Purity: | n/a | ||
Prep. Method: | n/a | ||
Name: | Lysozyme (turkey egg) | ||
Synonyms: | LYSC_MELGA | LYZ | turkey egg lysozyme (TEL) | ||
Type: | Enzyme | ||
Mol. Mass.: | 16145.52 | ||
Organism: | Meleagris gallopavo | ||
Description: | P00703 | ||
Residue: | 147 | ||
Sequence: |
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