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| Reaction Details | |||
|---|---|---|---|
 | Report a problem with these data | ||
| Cell Reactant: | Quinone Reductase 2 (QR2) | ||
| Syringe Reactant: | BDBM29611 | ||
| Meas. Tech.: | Isothermal Titration Calorimetry | ||
| Entry Date: | 06/10/09 | ||
| ΔG°: | -8.5±0.380 (kcal/mole) | ||
| pH: | 7.5±n/a | ||
| Log10Kb: | 6.15± 5.72 | ||
| Temperature: | 298.15±n/a (K) | ||
| ΔH° : | n/a | ||
| ΔHobs : | -7.85±0.620 (kcal/mole) | ||
| Ionic Strength: | n/a | ||
| no | |||
| Protons Released: | n/a | ||
| ΔCp : | n/a | ||
| Stoich. Param.: | 0.8 | ||
| ΔS° : | 0±0 (kcal/mole-K) | ||
| Comments: | n/a | ||
| Citation |  Calamini, B; Santarsiero, BD; Boutin, JA; Mesecar, AD Kinetic, thermodynamic and X-ray structural insights into the interaction of melatonin and analogues with quinone reductase 2. Biochem J413:81-91 (2008) [PubMed] Article | ||
| More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
| Quinone Reductase 2 (QR2) | |||
| Source: | Human QR2 was expressed and purified from E. coli cells. | ||
| Purity: | n/a | ||
| Prep. Method: | The enzyme was further applied to a Hiload 26/60 Superdex 75 (GE Healthcare) column. The fractions containing pure enzyme were pooled and finally applied to a 10/100 GL MonoQ (GE Healthcare) strong anion-exchange column. The protein was eluted using a gradient. The purest protein fractions were pooled, buffer-exchanged and concentrated. Samples of QR2 were extensively dialysed against ITC buffer, and degassed before assay. | ||
| Name: | Ribosyldihydronicotinamide dehydrogenase [quinone] | ||
| Synonyms: | Metallothionein-3 | NMOR2 | NQO2 | NQO2_HUMAN | NRH dehydrogenase [quinone] 2 | NRH:quinone oxidoreductase 2 | QR2 | Quinone reductase 2 | Quinone reductase 2 (NQO2) | Ribosyldihydronicotinamide dehydrogenase [quinone] | ||
| Type: | Protein | ||
| Mol. Mass.: | 25917.25 | ||
| Organism: | Homo sapiens (Human) | ||
| Description: | P16083 | ||
| Residue: | 231 | ||
| Sequence: |
| ||
| BDBM29611 | |||
| Source: | n/a | ||
| Purity: | n/a | ||
| Prep. Method: | n/a | ||
| Name | BDBM29611 | ||
| Synonyms: | 2-Iodomelatonin | CHEMBL289233 | Melatonin,2-Iodo | ||
| Type | Small organic molecule | ||
| Emp. Form. | C13H15IN2O2 | ||
| Mol. Mass. | 358.1749 | ||
| SMILES | COc1ccc2[nH]c(I)c(CCNC(C)=O)c2c1 | ||
| Structure | 
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