Reaction Details | |||
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Cell Reactant: | HIV-1 Protease Mutant (V82A/I84V) | ||
Syringe Reactant: | BDBM581 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 05/10/04 | ||
ΔG°: | -8± (kcal/mole) | ||
pH: | 5±n/a | ||
Log10Kb: | 5.89± 4.70 | ||
Temperature: | 298.15±n/a (K) | ||
ΔH° : | 10.4±0.200 (kcal/mole) | ||
ΔHobs : | 10.4±0.200 (kcal/mole) | ||
Ionic Strength: | n/a | ||
not known | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | 0.0600± (kcal/mole-K) | ||
Comments: | n/a | ||
Citation | Ohtaka, H; Schön, A; Freire, E Multidrug resistance to HIV-1 protease inhibition requires cooperative coupling between distal mutations. Biochemistry42:13659-66 (2003) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Data Fit Method , Instrument Info | ||
HIV-1 Protease Mutant (V82A/I84V) | |||
Source: | The genes encoding HIV-1 protease mutants and wild type were expressed in E. coli. | ||
Purity: | 99% | ||
Prep. Method: | HIV-1 protease was purified and refolded from E. coli inclusion bodies. | ||
Name: | HIV-1 Protease Mutant (V82A/I84V) | ||
Synonyms: | n/a | ||
Type: | Protein Complex | ||
Mol. Mass.: | n/a | ||
Description: | n/a | ||
Components: | This complex has 2 components. | ||
Component 1 | |||
Name: | HIV-1 Protease Mutant (V82A/I84V) chain A | ||
Synonyms: | HIV-1 Protease Mutant (Q7K/L33I/L63I/V82A/I84V) | HIV-1 Protease Mutant (V82A/I84V) chain B | ||
Type: | Enzyme Subunit | ||
Mol. Mass.: | 10754.16 | ||
Organism: | Human immunodeficiency virus type 1 | ||
Description: | Mutations at selected positions (V82A/I84V) were introduced into HIV-1 protease pseudo wild type (Q7K/L33I/L63I) | ||
Residue: | 99 | ||
Sequence: |
| ||
Component 2 | |||
Name: | HIV-1 Protease Mutant (V82A/I84V) chain A | ||
Synonyms: | HIV-1 Protease Mutant (Q7K/L33I/L63I/V82A/I84V) | HIV-1 Protease Mutant (V82A/I84V) chain B | ||
Type: | Enzyme Subunit | ||
Mol. Mass.: | 10754.16 | ||
Organism: | Human immunodeficiency virus type 1 | ||
Description: | Mutations at selected positions (V82A/I84V) were introduced into HIV-1 protease pseudo wild type (Q7K/L33I/L63I) | ||
Residue: | 99 | ||
Sequence: |
| ||
BDBM581 | |||
Source: | Bachem AG | ||
Purity: | n/a | ||
Prep. Method: | Compound was prepared as a 7.8 mM stock solution in 9 mM NaOH. | ||
Name | BDBM581 | ||
Synonyms: | 4-{2-[(3R)-4-[2-(2-acetamido-3-methylbutanamido)-3-methylbutanamido]-3-hydroxy-6-methylheptanamido]propanamido}-3-hydroxy-6-methylheptanoic acid | Acetyl Pepstatin | ||
Type | Small organic molecule | ||
Emp. Form. | C31H57N5O9 | ||
Mol. Mass. | 643.8124 | ||
SMILES | CC(C)CC(NC(=O)C(C)NC(=O)C[C@@H](O)C(CC(C)C)NC(=O)C(NC(=O)C(NC(C)=O)C(C)C)C(C)C)C(O)CC(O)=O |r| | ||
Structure |
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