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Cell Reactant:HIV-1 Protease Mutant (V82A/I84V)
Syringe Reactant:BDBM581
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:05/10/04
 
ΔG°:-8± (kcal/mole)
pH:5±n/a
Log10Kb:5.89± 4.70
Temperature:298.15±n/a (K)
ΔH° :10.4±0.200 (kcal/mole)
ΔHobs :10.4±0.200 (kcal/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:not known
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:n/a
ΔS° :0.0600± (kcal/mole-K)
Comments:n/a
 
Citation Ohtaka, HSchön, AFreire, E Multidrug resistance to HIV-1 protease inhibition requires cooperative coupling between distal mutations. Biochemistry42:13659-66 (2003) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
HIV-1 Protease Mutant (V82A/I84V)
Source:The genes encoding HIV-1 protease mutants and wild type were expressed in E. coli.
Purity:99%
Prep. Method:HIV-1 protease was purified and refolded from E. coli inclusion bodies.
Name:HIV-1 Protease Mutant (V82A/I84V)
Synonyms:n/a
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:HIV-1 Protease Mutant (V82A/I84V) chain A
Synonyms:HIV-1 Protease Mutant (Q7K/L33I/L63I/V82A/I84V) | HIV-1 Protease Mutant (V82A/I84V) chain B
Type:Enzyme Subunit
Mol. Mass.:10754.16
Organism:Human immunodeficiency virus type 1
Description:Mutations at selected positions (V82A/I84V) were introduced into HIV-1 protease pseudo wild type (Q7K/L33I/L63I)
Residue:99
Sequence:
PQITLWKRPLVTIKIGGQLKEALLDTGADDTVIEEMSLPGRWKPKMIGGIGGFIKVRQYD
QIIIEICGHKAIGTVLVGPTPANVIGRNLLTQIGCTLNF
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Component 2
Name:HIV-1 Protease Mutant (V82A/I84V) chain A
Synonyms:HIV-1 Protease Mutant (Q7K/L33I/L63I/V82A/I84V) | HIV-1 Protease Mutant (V82A/I84V) chain B
Type:Enzyme Subunit
Mol. Mass.:10754.16
Organism:Human immunodeficiency virus type 1
Description:Mutations at selected positions (V82A/I84V) were introduced into HIV-1 protease pseudo wild type (Q7K/L33I/L63I)
Residue:99
Sequence:
PQITLWKRPLVTIKIGGQLKEALLDTGADDTVIEEMSLPGRWKPKMIGGIGGFIKVRQYD
QIIIEICGHKAIGTVLVGPTPANVIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM581
Source:Bachem AG
Purity:n/a
Prep. Method:Compound was prepared as a 7.8 mM stock solution in 9 mM NaOH.
NameBDBM581
Synonyms:4-{2-[(3R)-4-[2-(2-acetamido-3-methylbutanamido)-3-methylbutanamido]-3-hydroxy-6-methylheptanamido]propanamido}-3-hydroxy-6-methylheptanoic acid | Acetyl Pepstatin
TypeSmall organic molecule
Emp. Form.C31H57N5O9
Mol. Mass.643.8124
SMILESCC(C)CC(NC(=O)C(C)NC(=O)C[C@@H](O)C(CC(C)C)NC(=O)C(NC(=O)C(NC(C)=O)C(C)C)C(C)C)C(O)CC(O)=O |r|
Structure
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