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Cell Reactant:Heat Shock Protein 90 (Hsp90)
Syringe Reactant:BDBM18134
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:09/29/09
 
ΔG°:-5.62± (kcal/mole)
pH:8±n/a
Log10Kb:4.36± 3.00
Temperature:281.15±n/a (K)
ΔH° :n/a
ΔHobs :-9.19±0.0500 (kcal/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:yes
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:n/a
ΔS° :-0.0100± (kcal/mole-K)
Comments:n/a
 
Citation Nilapwar, SWilliams, EFu, CProdromou, CPearl, LHWilliams, MALadbury, JE Structural-thermodynamic relationships of interactions in the N-terminal ATP-binding domain of Hsp90. J Mol Biol392:923-36 (2009) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
Heat Shock Protein 90 (Hsp90)
Source:His-tagged N-terminal domain of yeast HSP90 was expressed and purified.
Purity:n/a
Prep. Method:Protein was purified by using nickel affinity column, and was subsequently concentrated by an (NH4)2SO4 precipitation and purified further using gel filtration chromatography.
Name:Heat Shock Protein 90 (Hsp90)
Synonyms:82 kDa heat shock protein | ATP-dependent molecular chaperone HSP82 | HSP82 | HSP82_YEAST | HSP90 | Heat shock protein Hsp90 heat-inducible isoform
Type:N-terminal domain
Mol. Mass.:23389.88
Organism:Saccharomyces cerevisiae (Baker s yeast)
Description:P02829 aa 1-207
Residue:207
Sequence:
MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEP
DLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQF
GVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDD
QLEYLEEKRIKEVIKRHSEFVAYPIQL
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  Blast E-value cutoff:
BDBM18134
Source:n/a
Purity:n/a
Prep. Method:n/a
NameBDBM18134
Synonyms:({[({[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl)oxy](hydroxy)phosphoryl}amino)phosphonic acid | 5-Adenylyl imidodiphosphate | AMP.P[NH]P | AMPPNP
TypeNucleoside or nucleotide
Emp. Form.C10H17N6O12P3
Mol. Mass.506.1963
SMILESNc1ncnc2n(cnc12)[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)NP(O)(O)=O)[C@@H](O)[C@H]1O |r|
Structure
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