Reaction Details | |||
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Cell Reactant: | HIV-1 Protease A Subtype | ||
Syringe Reactant: | BDBM520 | ||
Meas. Tech.: | Isothermal Titration Calorimetry | ||
Entry Date: | 06/22/04 | ||
ΔG°: | -55.2± (kJ/mole) | ||
pH: | 5±n/a | ||
Log10Kb: | 9.65± 8 | ||
Temperature: | 298.15±n/a (K) | ||
ΔH° : | -12.1± (kJ/mole) | ||
ΔHobs : | -12.1± (kJ/mole) | ||
Ionic Strength: | n/a | ||
not known | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | n/a | ||
ΔS° : | 0.1445± (kJ/mole-K) | ||
Comments: | In displacement titration, inhibitors were injected into the calorimetric cell containing the protein prebound to a weak inhibitor acetylpepstatin. | ||
Citation | Velazquez-Campoy, A; Vega, S; Freire, E Amplification of the effects of drug resistance mutations by background polymorphisms in HIV-1 protease from African subtypes. Biochemistry41:8613-9 (2002) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
HIV-1 Protease A Subtype | |||
Source: | Plasmid-encoded HIV-1 protease was expressed in E. coli cells. | ||
Purity: | 99% | ||
Prep. Method: | HIV-1 protease was purified and refolded from E. coli inclusion bodies. | ||
Name: | HIV-1 Protease A Subtype | ||
Synonyms: | n/a | ||
Type: | Protein Complex | ||
Mol. Mass.: | n/a | ||
Description: | n/a | ||
Components: | This complex has 2 components. | ||
Component 1 | |||
Name: | HIV-1 Protease A Subtype chain A | ||
Synonyms: | HIV-1 Protease A Subtype chain B | HIV-1 Protease Mutant (Q7K/I13V/E35D/M36I/S37N/R41K/R57K/H69K/L89M) | ||
Type: | Enzyme Subunit | ||
Mol. Mass.: | 10716.69 | ||
Organism: | Human immunodeficiency virus type 1 | ||
Description: | n/a | ||
Residue: | 99 | ||
Sequence: |
| ||
Component 2 | |||
Name: | HIV-1 Protease A Subtype chain A | ||
Synonyms: | HIV-1 Protease A Subtype chain B | HIV-1 Protease Mutant (Q7K/I13V/E35D/M36I/S37N/R41K/R57K/H69K/L89M) | ||
Type: | Enzyme Subunit | ||
Mol. Mass.: | 10716.69 | ||
Organism: | Human immunodeficiency virus type 1 | ||
Description: | n/a | ||
Residue: | 99 | ||
Sequence: |
| ||
BDBM520 | |||
Source: | Purified from commercial capsules | ||
Purity: | n/a | ||
Prep. Method: | Further purified by HPLC using a semipreparative C-18 reversed-phase column developed with 0-100% acetonitrile in 0.05% TFA | ||
Name | BDBM520 | ||
Synonyms: | 1,3-thiazol-5-ylmethyl N-[(2S,3S,5S)-3-hydroxy-5-[(2S)-3-methyl-2-{[methyl({[2-(propan-2-yl)-1,3-thiazol-4-yl]methyl})carbamoyl]amino}butanamido]-1,6-diphenylhexan-2-yl]carbamate | ABT-538 | CHEMBL163 | Norvir | RTV | Ritonavir | ||
Type | Small organic molecule | ||
Emp. Form. | C37H48N6O5S2 | ||
Mol. Mass. | 720.944 | ||
SMILES | CC(C)[C@H](NC(=O)N(C)Cc1csc(n1)C(C)C)C(=O)N[C@H](C[C@H](O)[C@H](Cc1ccccc1)NC(=O)OCc1cncs1)Cc1ccccc1 | ||
Structure |
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