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Cell Reactant:HIV-1 Protease B Subtype Mutant (V82F/I84V)
Syringe Reactant:BDBM518
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:06/22/04
 
ΔG°:-11.3± (kcal/mole)
pH:5±n/a
Log10Kb:8.28± 6.55
Temperature:298.15±n/a (K)
ΔH° :4.40± (kcal/mole)
ΔHobs :4.40± (kcal/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:not known
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:n/a
ΔS° :0.0500± (kcal/mole-K)
Comments:In displacement titration, inhibitors were injected into the calorimetric cell containing the protein prebound to a weak inhibitor acetylpepstatin.
 
Citation Velazquez-Campoy, AVega, SFreire, E Amplification of the effects of drug resistance mutations by background polymorphisms in HIV-1 protease from African subtypes. Biochemistry41:8613-9 (2002) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Instrument Info
 
HIV-1 Protease B Subtype Mutant (V82F/I84V)
Source:Plasmid-encoded HIV-1 protease was expressed in E. coli cells.
Purity:99%
Prep. Method:HIV-1 protease was purified and refolded from E. coli inclusion bodies.
Name:HIV-1 Protease B Subtype Mutant (V82F/I84V)
Synonyms:n/a
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:HIV-1 Protease B Subtype Mutant (V82F/I84V) chain A
Synonyms:HIV-1 Protease B Subtype Mutant (V82F/I84V) chain B | HIV-1 Protease Mutant (Q7K/V82F/I84V)
Type:Enzyme Subunit
Mol. Mass.:10816.23
Organism:Human immunodeficiency virus type 1
Description:n/a
Residue:99
Sequence:
PQVTLWKRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPFNVIGRNLLTQIGCTLNF
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  Blast E-value cutoff:
Component 2
Name:HIV-1 Protease B Subtype Mutant (V82F/I84V) chain A
Synonyms:HIV-1 Protease B Subtype Mutant (V82F/I84V) chain B | HIV-1 Protease Mutant (Q7K/V82F/I84V)
Type:Enzyme Subunit
Mol. Mass.:10816.23
Organism:Human immunodeficiency virus type 1
Description:n/a
Residue:99
Sequence:
PQVTLWKRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPFNVIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM518
Source:Purified from commercial capsules
Purity:n/a
Prep. Method:Further purified by HPLC using a semipreparative C-18 reversed-phase column developed with 0-100% acetonitrile in 0.05% TFA
NameBDBM518
Synonyms:(3S,4aS,8aS)-N-tert-butyl-2-[(2R,3R)-2-hydroxy-3-[(3-hydroxy-2-methylphenyl)formamido]-4-(phenylsulfanyl)butyl]-decahydroisoquinoline-3-carboxamide | AG-1343 | CHEMBL584 | Nelfinavir | Viracept
TypeSmall organic molecule
Emp. Form.C32H45N3O4S
Mol. Mass.567.782
SMILESCc1c(O)cccc1C(=O)N[C@@H](CSc1ccccc1)[C@H](O)CN1C[C@H]2CCCC[C@H]2C[C@H]1C(=O)NC(C)(C)C |@@:23|
Structure
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