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TargetDimer of Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,D531N]
LigandBDBM517
Substrate/CompetitorHIV Peptide Substrate
Meas. Tech.Protease Inhibition Assay
pH5.6±n/a
Temperature310.15±n/a K
Ki 7.0±1.0 nM
Km31000±5000 nM
CommentsKcat/Km=7±1 min-1nM-1
Citation Kovalevsky, AYTie, YLiu, FBoross, PIWang, YFLeshchenko, SGhosh, AKHarrison, RWWeber, IT Effectiveness of nonpeptide clinical inhibitor TMC-114 on HIV-1 protease with highly drug resistant mutations D30N, I50V, and L90M. J Med Chem49:1379-87 (2006) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Dimer of Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,D531N]
Name:Dimer of Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,D531N]
Synonyms:HIV-1 Protease Mutant (D30N)
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,D531N]
Synonyms:HIV-1 Protease Mutant (D30N) chain A | HIV-1 Protease Mutant (D30N) chain B | LAI(D30N) | POL_HV1BR | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10732.13
Organism:Human immunodeficiency virus type 1
Description:P03367[501-599,Q508K,L534I,L564I,C568A,C596A,D531N]
Residue:99
Sequence:
PQITLWKRPLVTIKIGGQLKEALLDTGADNTVIEEMSLPGRWKPKMIGGIGGFIKVRQYD
QIIIEIAGHKAIGTVLVGPTPVNIIGRNLLTQIGATLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
Component 2
Name:Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,D531N]
Synonyms:HIV-1 Protease Mutant (D30N) chain A | HIV-1 Protease Mutant (D30N) chain B | LAI(D30N) | POL_HV1BR | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10732.13
Organism:Human immunodeficiency virus type 1
Description:P03367[501-599,Q508K,L534I,L564I,C568A,C596A,D531N]
Residue:99
Sequence:
PQITLWKRPLVTIKIGGQLKEALLDTGADNTVIEEMSLPGRWKPKMIGGIGGFIKVRQYD
QIIIEIAGHKAIGTVLVGPTPVNIIGRNLLTQIGATLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM517
HIV Peptide Substrate
Name:HIV Peptide Substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:3156.95
Organism:n/a
Description:n/a
Residue:27
Sequence:
Ac-RE(Edans)SQNY*PIVRK(Dabcyl)R-CO-NH2