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TargetHIV-1 Protease
LigandBDBM9183
Substrate/CompetitorHIV Protease substrate
Meas. Tech.Protease Inhibition Assay
pH6.4±n/a
Temperature298.15±n/a K
Ki 0.05±n/a nM
IC50 720±110 nM
Citation Sherrill RGAndrews CWBock WJDavis-Ward RGFurfine ESHazen RJRutkowske RDSpaltenstein AWright LL Optimization of pyrrolidinone based HIV protease inhibitors. Bioorg Med Chem Lett 15:81-4 (2005) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
HIV-1 Protease
Name:HIV-1 Protease
Synonyms:n/a
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:HIV-1 Protease chain A
Synonyms:n/a
Type:Enzyme Subunit
Mol. Mass.:10732.11
Organism:Human immunodeficiency virus type 1
Description:The HIV-1 protease (Genbank HIVHXB2CG) clone was constructed with the substitutions Q7K, L33I, and L63I, to minimize the autoproteolysis of the protease, and C67A and C95A, to prevent cysteine-thiol oxidation.
Residue:99
Sequence:
PQITLWKRPLVTIKIGGQLKEALLDTGADDTVIEEMSLPGRWKPKMIGGIGGFIKVRQYD
QIIIEIAGHKAIGTVLVGPTPVNIIGRNLLTQIGATLNF
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Component 2
Name:HIV-1 Protease chain A
Synonyms:n/a
Type:Enzyme Subunit
Mol. Mass.:10732.11
Organism:Human immunodeficiency virus type 1
Description:The HIV-1 protease (Genbank HIVHXB2CG) clone was constructed with the substitutions Q7K, L33I, and L63I, to minimize the autoproteolysis of the protease, and C67A and C95A, to prevent cysteine-thiol oxidation.
Residue:99
Sequence:
PQITLWKRPLVTIKIGGQLKEALLDTGADDTVIEEMSLPGRWKPKMIGGIGGFIKVRQYD
QIIIEIAGHKAIGTVLVGPTPVNIIGRNLLTQIGATLNF
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  Blast E-value cutoff:
BDBM9183
HIV Protease substrate
Name:HIV Protease substrate
Synonyms:Fluorogenic Peptide Substrate
Type:Peptide
Mol. Mass.:3467.28
Organism:n/a
Description:n/a
Residue:30
Sequence:
2-(aminobenzoyl)-Thr-Ile-Nle-Phe(p-NO2)-Gln-ArgNH2