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TargetGag-Pol polyprotein [600-1027,K702N,Y780C]/[600-1155,K702N,Y780C]
LigandBDBM10236
Substrate/Competitorpoly rC/biotin-dG15
Meas. Tech.HIV-1 RT Assay
pH7.4±n/a
Temperature310.15±n/a K
IC50 280±n/a nM
Citation Yoakim, C; Bonneau, PR; Deziel, R; Doyon, L; Duan, J; Guse, I; Landry, S; Malenfant, E; Naud, J; Ogilvie, WW; O'Meara, JA; Plante, R; Simoneau, B; Thavonekham, B; Bos, M; Cordingley, MG Novel nevirapine-like inhibitors with improved activity against NNRTI-resistant HIV: 8-heteroarylthiomethyldipyridodiazepinone derivatives. Bioorg Med Chem Lett14:739-42 (2004) [PubMed]  Article
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Gag-Pol polyprotein [600-1027,K702N,Y780C]/[600-1155,K702N,Y780C]
Name:Gag-Pol polyprotein [600-1027,K702N,Y780C]/[600-1155,K702N,Y780C]
Synonyms:HIV-1 Reverse Transcriptase Mutant (K103N/Y181C)
Type:Protein Complex
Mol. Mass.:n/a
Description:n/a
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [600-1155,K702N,Y780C]
Synonyms:HIV-1 Reverse Transcriptase Mutant (K103N/Y181C) Chain A | POL_HV1B1 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:63853.70
Organism:Human immunodeficiency virus type 1
Description:P03366[600-1155,K702N,Y780C]
Residue:556
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKNKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
CQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGI
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Component 2
Name:Gag-Pol polyprotein [600-1027,K702N,Y780C]
Synonyms:HIV-1 Reverse Transcriptase Mutant (K103N/Y181C) Chain B | POL_HV1B1 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:49893.53
Organism:Human immunodeficiency virus type 1
Description:P03366[600-1027,K702N,Y780C]
Residue:428
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKNKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
CQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVKQLCKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQ
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BDBM10236
poly rC/biotin-dG15
Name:poly rC/biotin-dG15
Synonyms:n/a
Type:RNA-DNA duplex
Mol. Mass.:358.43
Organism:n/a
Description:The incorporation of Tritium-labeled [3H]dGTP onto a (poly)rC:(biotin)dG template:primer.
Residue:3
Sequence:
n/a