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TargetATP-dependent RNA helicase RhlE
LigandBDBM13321
Substrate/Competitorbiotinylated lamin B peptide substrate
Meas. Tech.PfPFT and Rat PFT IC50 Determination
pH7.5±n/a
Temperature310.15±n/a K
IC50 0.4±n/a nM
Citation Nallan, L; Bauer, KD; Bendale, P; Rivas, K; Yokoyama, K; Horney, CP; Pendyala, PR; Floyd, D; Lombardo, LJ; Williams, DK; Hamilton, A; Sebti, S; Windsor, WT; Weber, PC; Buckner, FS; Chakrabarti, D; Gelb, MH; Van Voorhis, WC Protein farnesyltransferase inhibitors exhibit potent antimalarial activity. J Med Chem48:3704-13 (2005) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
ATP-dependent RNA helicase RhlE
Name:ATP-dependent RNA helicase RhlE
Synonyms:Protein Farnesyltransferase (PFT) | Protein prenyltransferase, alpha subunit
Type:Enzyme
Mol. Mass.:54781.31
Organism:Plasmodium falciparum (malaria parasite P. falciparum)
Description:Q5E175
Residue:500
Sequence:
MGFTSLGLSAPILKAVEEQGYSTPSPIQLQAIPAVIEGKDVMAAAQTGTGKTAGFTLPLL
ERLSNGPKRKFNQVRALVLTPTRELAAQVHESVEKYSKNLPLTSDVVFGGVKVNPQMQRL
RRGVDVLVATPGRLLDLANQNAIKFDQLEILVLDEADRMLDMGFIHDIKKILAKLPKNRQ
NLLFSATFSDEIRQLAKGLVKDPVEISVAKRNTTAETVEQSVYVMDKGRKPKVLTKLIKD
NDWKQVLVFSKTKHGANRLAKTLEEKGVSAAAIHGNKSQGARTKALANFKSGQVRVLVAT
DIAARGLDIEQLPQVINVDLPKVPEDYVHRIGRTGRAGATGKAISFVSEDEAKELFAIER
LIQKVLPRHVLEGFEPVNKVPESKLDTRPIKPKKPKKPKAPRVEHKDGQRSGENRNGNKQ
GAKQGQKPATKRTPTNNPSGKKEGTDSDKKKRPFSGKPKTKGTGENRGNGSNFGKSKSTP
KSDVKPRRQGPRPARKPKAN
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM13321
biotinylated lamin B peptide substrate
Name:biotinylated lamin B peptide substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:1715.01
Organism:n/a
Description:A human lamin-B carboxy-terminus sequence peptide (biotin-YRASNRSCAIM) is 3H-farnesylated at the cysteine residue when processed by farnesyltransferase.
Residue:15
Sequence:
biotin-YRASNRSCAIM