Reaction Details |
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Target | Dimer of Gag-Pol polyprotein [501-599,V583F] |
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Ligand | BDBM520 |
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Substrate/Competitor | HIV Protease Chromogenic Peptide Substrate |
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Meas. Tech. | Protease Inhibition Assay |
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pH | 4.7±n/a |
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Temperature | 310.15±n/a K |
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Ki | 4±1 nM |
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Km | 18000±2000 nM |
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Comments | kcat/Km=0.40 +/- 0.05 uM-1sec-1 |
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Citation | Clemente, JC; Coman, RM; Thiaville, MM; Janka, LK; Jeung, JA; Nukoolkarn, S; Govindasamy, L; Agbandje-McKenna, M; McKenna, R; Leelamanit, W; Goodenow, MM; Dunn, BM Analysis of HIV-1 CRF_01 A/E protease inhibitor resistance: structural determinants for maintaining sensitivity and developing resistance to atazanavir. Biochemistry45:5468-77 (2006) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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Dimer of Gag-Pol polyprotein [501-599,V583F] |
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Name: | Dimer of Gag-Pol polyprotein [501-599,V583F] |
Synonyms: | HIV-1 Protease B Subtype Mutant (V82F) |
Type: | Protein Complex |
Mol. Mass.: | n/a |
Description: | HIV-1 protease DNA for all the HIV B and AE variants was cloned, and protein was expressed and purified from Escherichia coli strain BL21. |
Components: | This complex has 2 components. |
Component 1 |
Name: | Gag-Pol polyprotein [501-599,V583F] |
Synonyms: | HIV-1 Protease B Subtype Mutant (V82F) Chain A | HIV-1 Protease B Subtype Mutant (V82F) Chain B | POL_HV1BR | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10843.23 |
Organism: | Human immunodeficiency virus type 1 |
Description: | P03367[501-599,V583F] |
Residue: | 99 |
Sequence: | PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPFNIIGRNLLTQIGCTLNF
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Component 2 |
Name: | Gag-Pol polyprotein [501-599,V583F] |
Synonyms: | HIV-1 Protease B Subtype Mutant (V82F) Chain A | HIV-1 Protease B Subtype Mutant (V82F) Chain B | POL_HV1BR | gag-pol |
Type: | Enzyme Subunit |
Mol. Mass.: | 10843.23 |
Organism: | Human immunodeficiency virus type 1 |
Description: | P03367[501-599,V583F] |
Residue: | 99 |
Sequence: | PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPFNIIGRNLLTQIGCTLNF
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BDBM520 |
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HIV Protease Chromogenic Peptide Substrate |
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Name: | HIV Protease Chromogenic Peptide Substrate |
Synonyms: | n/a |
Type: | Peptide |
Mol. Mass.: | 1420.11 |
Organism: | n/a |
Description: | n/a |
Residue: | 13 |
Sequence: | |