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TargetDimer of Gag-Pol polyprotein [501-599,V583F]
LigandBDBM520
Substrate/CompetitorHIV Protease Chromogenic Peptide Substrate
Meas. Tech.Protease Inhibition Assay
pH4.7±n/a
Temperature310.15±n/a K
Ki 4±1 nM
Km18000±2000 nM
Commentskcat/Km=0.40 +/- 0.05 uM-1sec-1
Citation Clemente, JCComan, RMThiaville, MMJanka, LKJeung, JANukoolkarn, SGovindasamy, LAgbandje-McKenna, MMcKenna, RLeelamanit, WGoodenow, MMDunn, BM Analysis of HIV-1 CRF_01 A/E protease inhibitor resistance: structural determinants for maintaining sensitivity and developing resistance to atazanavir. Biochemistry45:5468-77 (2006) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Dimer of Gag-Pol polyprotein [501-599,V583F]
Name:Dimer of Gag-Pol polyprotein [501-599,V583F]
Synonyms:HIV-1 Protease B Subtype Mutant (V82F)
Type:Protein Complex
Mol. Mass.:n/a
Description:HIV-1 protease DNA for all the HIV B and AE variants was cloned, and protein was expressed and purified from Escherichia coli strain BL21.
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [501-599,V583F]
Synonyms:HIV-1 Protease B Subtype Mutant (V82F) Chain A | HIV-1 Protease B Subtype Mutant (V82F) Chain B | POL_HV1BR | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10843.23
Organism:Human immunodeficiency virus type 1
Description:P03367[501-599,V583F]
Residue:99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPFNIIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
Component 2
Name:Gag-Pol polyprotein [501-599,V583F]
Synonyms:HIV-1 Protease B Subtype Mutant (V82F) Chain A | HIV-1 Protease B Subtype Mutant (V82F) Chain B | POL_HV1BR | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10843.23
Organism:Human immunodeficiency virus type 1
Description:P03367[501-599,V583F]
Residue:99
Sequence:
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYD
QILIEICGHKAIGTVLVGPTPFNIIGRNLLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM520
HIV Protease Chromogenic Peptide Substrate
Name:HIV Protease Chromogenic Peptide Substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:1420.11
Organism:n/a
Description:n/a
Residue:13
Sequence:
K-A-R-V-L*Nph-E-A-nL-G