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TargetHIV-1 Protease AE Mutant (V82F)
LigandBDBM520
Substrate/CompetitorHIV Protease Chromogenic Peptide Substrate
Meas. Tech.Protease Inhibition Assay
Ki 3.1±n/a nM
Citation Clemente JCComan RMThiaville MMJanka LKJeung JANukoolkarn SGovindasamy LAgbandje-McKenna MMcKenna RLeelamanit WGoodenow MMDunn BM Analysis of HIV-1 CRF_01 A/E protease inhibitor resistance: structural determinants for maintaining sensitivity and developing resistance to atazanavir. Biochemistry 45:5468-77 (2006) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
HIV-1 Protease AE Mutant (V82F)
Name:HIV-1 Protease AE Mutant (V82F)
Synonyms:HIV-1 Protease Mutant (V3I, I13V, E35D, M36I, S37N, R41K, H69K, V82F, L89M) | Pretherapy isolated AE protease mutant (V82F)
Type:Protein Complex
Mol. Mass.:n/a
Description:HIV-1 protease DNA for all the HIV B and AE variants was cloned, and protein was expressed and purified from Escherichia coli strain BL21.
Components:This complex has 2 components.
Component 1
Name:HIV-1 Protease AE Mutant (V82F) Chain A
Synonyms:HIV-1 Protease AE Mutant (V82F) Chain B
Type:Enzyme Subunit
Mol. Mass.:10805.72
Organism:Human immunodeficiency virus type 1
Description:n/a
Residue:99
Sequence:
PQITLWQRPLVTVKIGGQLKEALLDTGADDTVLEDINLPGKWKPKMIGGIGGFIKVRQYD
QILIEICGKKAIGTVLVGPTPFNIIGRNMLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
Component 2
Name:HIV-1 Protease AE Mutant (V82F) Chain A
Synonyms:HIV-1 Protease AE Mutant (V82F) Chain B
Type:Enzyme Subunit
Mol. Mass.:10805.72
Organism:Human immunodeficiency virus type 1
Description:n/a
Residue:99
Sequence:
PQITLWQRPLVTVKIGGQLKEALLDTGADDTVLEDINLPGKWKPKMIGGIGGFIKVRQYD
QILIEICGKKAIGTVLVGPTPFNIIGRNMLTQIGCTLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM520
HIV Protease Chromogenic Peptide Substrate
Name:HIV Protease Chromogenic Peptide Substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:1420.11
Organism:n/a
Description:n/a
Residue:13
Sequence:
K-A-R-V-L*Nph-E-A-nL-G