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TargetDimer of Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,I551V]
LigandBDBM13935
Substrate/CompetitorChromogenic Substrate
Meas. Tech.Protease Inhibition Assay
pH5±n/a
Temperature295.15±n/a K
Ki 230±n/a nM
Km500000±36000 nM
Commentskcat/Km=0.93 +/- 0.08 min-1uM-1
Citation Liu, F; Boross, PI; Wang, YF; Tozser, J; Louis, JM; Harrison, RW; Weber, IT Kinetic, stability, and structural changes in high-resolution crystal structures of HIV-1 protease with drug-resistant mutations L24I, I50V, and G73S. J Mol Biol354:789-800 (2005) [PubMed]  Article
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Dimer of Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,I551V]
Name:Dimer of Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,I551V]
Synonyms:HIV-1 Protease Mutant (I50V)
Type:Protein Complex
Mol. Mass.:n/a
Description:The HIV-1 PR DNA for all of wildtype and mutants was cloned, and protein was expressed, purified, and refolded from Escherichia coli strain BL21.
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,I551V]
Synonyms:HIV-1 Protease Mutant (I50V) chain A | HIV-1 Protease Mutant (I50V) chain B | POL_HV1BR | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10718.08
Organism:Human immunodeficiency virus type 1
Description:All of the mutant constructs in this study are on the wild type containing the substitutions Q7K, L33I, and L63I, to minimize the autoproteolysis of the protease, and C67A and C95A, to prevent cysteine-thiol oxidation.
Residue:99
Sequence:
PQITLWKRPLVTIKIGGQLKEALLDTGADDTVIEEMSLPGRWKPKMIGGVGGFIKVRQYD
QIIIEIAGHKAIGTVLVGPTPVNIIGRNLLTQIGATLNF
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  Blast E-value cutoff:
Component 2
Name:Gag-Pol polyprotein [501-599,Q508K,L534I,L564I,C568A,C596A,I551V]
Synonyms:HIV-1 Protease Mutant (I50V) chain A | HIV-1 Protease Mutant (I50V) chain B | POL_HV1BR | gag-pol
Type:Enzyme Subunit
Mol. Mass.:10718.08
Organism:Human immunodeficiency virus type 1
Description:All of the mutant constructs in this study are on the wild type containing the substitutions Q7K, L33I, and L63I, to minimize the autoproteolysis of the protease, and C67A and C95A, to prevent cysteine-thiol oxidation.
Residue:99
Sequence:
PQITLWKRPLVTIKIGGQLKEALLDTGADDTVIEEMSLPGRWKPKMIGGVGGFIKVRQYD
QIIIEIAGHKAIGTVLVGPTPVNIIGRNLLTQIGATLNF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM13935
Chromogenic Substrate
Name:Chromogenic Substrate
Synonyms:CA/p2#
Type:Peptide
Mol. Mass.:2887.67
Organism:n/a
Description:n/a
Residue:25
Sequence:
K-A-R-V-Nle-(p-nitroPhe)-E-A-Nle-amide