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TargetTyrosine-protein kinase Lck [245-498]
LigandBDBM14949
Substrate/CompetitorGastrin Peptide Substrate
Meas. Tech.Homogeneous Time-Resolved Fluorescence (HTRF) Enzyme Assay
IC50<1±n/a nM
Citation Cee, VJ; Albrecht, BK; Geuns-Meyer, S; Hughes, P; Bellon, S; Bready, J; Caenepeel, S; Chaffee, SC; Coxon, A; Emery, M; Fretland, J; Gallant, P; Gu, Y; Hodous, BL; Hoffman, D; Johnson, RE; Kendall, R; Kim, JL; Long, AM; McGowan, D; Morrison, M; Olivieri, PR; Patel, VF; Polverino, A; Powers, D; Rose, P; Wang, L; Zhao, H Alkynylpyrimidine amide derivatives as potent, selective, and orally active inhibitors of Tie-2 kinase. J Med Chem50:627-40 (2007) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Tyrosine-protein kinase Lck [245-498]
Name:Tyrosine-protein kinase Lck [245-498]
Synonyms:LCK | LCK_HUMAN | LSK | Lymphocyte cell-specific protein-tyrosine kinase | Proto-oncogene tyrosine-protein kinase LCK | T cell-specific protein-tyrosine kinase | p56-LCK
Type:Kinase domain
Mol. Mass.:29166.29
Organism:Homo sapiens (Human)
Description:Recombinant kinase domain (amino acid 245 to 498) fused with GST tag.
Residue:254
Sequence:
LKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLY
AVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRD
LRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVW
SFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDR
PTFDYLRSVLEDFF
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM14949
Gastrin Peptide Substrate
Name:Gastrin Peptide Substrate
Synonyms:Biotinylated gastrin peptide substrate | biotinylated gastrin substrate | gastrin substrate
Type:Peptide
Mol. Mass.:1823.88
Organism:n/a
Description:n/a
Residue:15
Sequence:
biotin-EEEEAYGWLDF