Reaction Details |
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Target | RAC-alpha serine/threonine-protein kinase [139-480,S378A,S381A,T450D,S473D] |
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Ligand | BDBM2579 |
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Substrate/Competitor | biotinylated Akt peptide substrate |
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Meas. Tech. | Akt Kinase Assay |
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pH | 7.5±n/a |
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Temperature | 295.15±n/a K |
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IC50 | 1.5±n/a nM |
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Citation | Li, Q; Woods, KW; Thomas, S; Zhu, GD; Packard, G; Fisher, J; Li, T; Gong, J; Dinges, J; Song, X; Abrams, J; Luo, Y; Johnson, EF; Shi, Y; Liu, X; Klinghofer, V; Des Jong, R; Oltersdorf, T; Stoll, VS; Jakob, CG; Rosenberg, SH; Giranda, VL Synthesis and structure-activity relationship of 3,4'-bispyridinylethylenes: discovery of a potent 3-isoquinolinylpyridine inhibitor of protein kinase B (PKB/Akt) for the treatment of cancer. Bioorg Med Chem Lett16:2000-7 (2006) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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RAC-alpha serine/threonine-protein kinase [139-480,S378A,S381A,T450D,S473D] |
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Name: | RAC-alpha serine/threonine-protein kinase [139-480,S378A,S381A,T450D,S473D] |
Synonyms: | AKT1 | AKT1_HUMAN | C-AKT | PKB | Protein kinase B (Akt 1) | Protein kinase B alpha | RAC | RAC-PK-alpha |
Type: | Enzyme |
Mol. Mass.: | 39513.28 |
Organism: | Homo sapiens (Human) |
Description: | Recombinant His-tagged Akt1 (S378A, S381A, T450D,
S473D; 139-480) |
Residue: | 342 |
Sequence: | AKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTE
NRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIV
SALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAP
EVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKA
LLAGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYF
DEEFTAQMITIDPPDQDDSMECVDSERRPHFPQFDYSASGTA
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BDBM2579 |
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biotinylated Akt peptide substrate |
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Name: | biotinylated Akt peptide substrate |
Synonyms: | n/a |
Type: | Biotinylated Peptide |
Mol. Mass.: | 3178.04 |
Organism: | n/a |
Description: | Km=15 uM |
Residue: | 28 |
Sequence: | biotin-Ahx-EELSPFRGRSRSAPPNLWAAQR
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