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TargetcAMP-dependent protein kinase catalytic subunit alpha
LigandBDBM15131
Substrate/CompetitorPKA-specific peptide substrate
Meas. Tech.PKA In Vitro Enzyme Assay
pH7.2±n/a
Temperature295.15±n/a K
IC50 27±n/a nM
Citation Davies, TG; Verdonk, ML; Graham, B; Saalau-Bethell, S; Hamlett, CC; McHardy, T; Collins, I; Garrett, MD; Workman, P; Woodhead, SJ; Jhoti, H; Barford, D A structural comparison of inhibitor binding to PKB, PKA and PKA-PKB chimera. J Mol Biol367:882-94 (2007) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
cAMP-dependent protein kinase catalytic subunit alpha
Name:cAMP-dependent protein kinase catalytic subunit alpha
Synonyms:KAPCA_BOVIN | PKA C-alpha | PKC | PRKACA | Protein Kinase C | Protein Kinase C, bovine brain | cAMP-Dependent Protein Kinase (PKA) | cAMP-dependent Protein Kinase, bovine heart | cAMP-dependent protein kinase A | cAMP-dependent protein kinase alpha-catalytic subunit | cAMP-dependent protein kinase, alpha-catalytic subunit
Type:Enzyme Complex
Mol. Mass.:40627.77
Organism:Bos taurus (bovine)
Description:The PKA holoenzyme purified from bovine heart, exists as an inactive tetrameric complex, which consists of a regulatory dimer associated with two catalytic subunits. It requires cAMP to activate the enzymatic reaction.
Residue:351
Sequence:
MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWENPAQNTAHLDQFERIKTLGTGSFGRVML
VKHMETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMV
MEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGY
IQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFF
ADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFAT
TDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF
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  Blast E-value cutoff:
BDBM15131
PKA-specific peptide substrate
Name:PKA-specific peptide substrate
Synonyms:PKAtide
Type:Peptide
Mol. Mass.:1019.14
Organism:n/a
Description:n/a
Residue:9
Sequence:
GRTGRRNSI