Ki Summary

Reaction Details

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Target

Dihydroorotate dehydrogenase (quinone), mitochondrial

Ligand

BDBM15354

Substrate/Competitor

BDBM15346

Meas. Tech.

DHODH Inhibition Assay

8±n/a

Temperature

295.15±n/a K

IC50

80±10 nM

Citation

Baldwin, J; Michnoff, CH; Malmquist, NA; White, J; Roth, MG; Rathod, PK; Phillips, MA High-throughput screening for potent and selective inhibitors of Plasmodium falciparum dihydroorotate dehydrogenase. J Biol Chem280:21847-53 (2005) [PubMed] Article

More Info.:

Get all data from this article, Inhibition_Run data, Solution Info, Assay Method

Dihydroorotate dehydrogenase (quinone), mitochondrial

Name:

Dihydroorotate dehydrogenase (quinone), mitochondrial

Synonyms:

DHOdehase | Dihydroorotate Dehydrogenase (DHODH) | Dihydroorotate dehydrogenase homolog | Dihydroorotate oxidase

Type:

Enzyme

Mol. Mass.:

65581.88

Organism:

Plasmodium falciparum

Description:

His6-tag DHODH was expressed in E. coli.

Residue:

569

Sequence:

MISKLKPQFMFLPKKHILSYCRKDVLNLFEQKFYYTSKRKESNNMKNESLLRLINYNRYY
NKIDSNNYYNGGKILSNDRQYIYSPLCEYKKKINDISSYVSVPFKINIRNLGTSNFVNNK
KDVLDNDYIYENIKKEKSKHKKIIFLLFVSLFGLYGFFESYNPEFFLYDIFLKFCLKYID
GEICHDLFLLLGKYNILPYDTSNDSIYACTNIKHLDFINPFGVAAGFDKNGVCIDSILKL
GFSFIEIGTITPRGQTGNAKPRIFRDVESRSIINSCGFNNMGCDKVTENLILFRKRQEED
KLLSKHIVGVSIGKNKDTVNIVDDLKYCINKIGRYADYIAINVSSPNTPGLRDNQEAGKL
KNIILSVKEEIDNLEKNNIMNDESTYNEDNKIVEKKNNFNKNNSHMMKDAKDNFLWFNTT
KKKPLVFVKLAPDLNQEQKKEIADVLLETNIDGMIISNTTTQINDIKSFENKKGGVSGAK
LKDISTKFICEMYNYTNKQIPIIASGGIFSGLDALEKIEAGASVCQLYSCLVFNGMKSAV
QIKRELNHLLYQRGYYNLKEAIGRKHSKS

BDBM15354

BDBM15346

Name

BDBM15354

Synonyms:

Asinex compound no. 9 | N-(3,4-dichlorophenyl)-2-methyl-3-nitrobenzamide

Type

Small organic molecule

Emp. Form.

C14H10Cl2N2O3

Mol. Mass.

325.147

SMILES

Cc1c(cccc1[N+]([O-])=O)C(=O)Nc1ccc(Cl)c(Cl)c1

Structure