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TargetBeta-secretase 1 [1-453]
LigandBDBM16048
Substrate/CompetitorBACE Peptide Substrate
Meas. Tech.Enzyme Inhibition Measurements
pH4.5±n/a
Temperature295.15±n/a K
IC50 12±n/a nM
Citation Hanessian, S; Yun, H; Hou, Y; Yang, G; Bayrakdarian, M; Therrien, E; Moitessier, N; Roggo, S; Veenstra, S; Tintelnot-Blomley, M; Rondeau, JM; Ostermeier, C; Strauss, A; Ramage, P; Paganetti, P; Neumann, U; Betschart, C Structure-based design, synthesis, and memapsin 2 (BACE) inhibitory activity of carbocyclic and heterocyclic peptidomimetics. J Med Chem48:5175-90 (2005) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Beta-secretase 1 [1-453]
Name:Beta-secretase 1 [1-453]
Synonyms:ASP2 | Asp 2 | Aspartyl protease 2 | BACE | BACE1 | BACE1_HUMAN | Beta-site APP cleaving enzyme 1 | Beta-site amyloid precursor protein cleaving enzyme 1 | KIAA1149 | Memapsin-2 | Membrane-associated aspartic protease 2 | beta-Secretase (BACE-1)
Type:Enzyme
Mol. Mass.:50193.81
Organism:Homo sapiens (Human)
Description:Recombinant human BACE (SwissProt accession number P56817, amino acids 1-453 + a thrombin-cleavable C-kappa tag) was produced in-house using Sf9 insect cells.
Residue:453
Sequence:
MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSF
VEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSST
YRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGIL
GLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGI
DHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKK
VFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRIT
ILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSAC
HVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDES
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM16048
BACE Peptide Substrate
Name:BACE Peptide Substrate
Synonyms:n/a
Type:Peptide
Mol. Mass.:1917.56
Organism:n/a
Description:Peptide substrates were obtained from Bachem (Bubendorf, Switzerland).
Residue:17
Sequence:
Mca-SEVNLDAEFK(DNP)-OH