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TargetCathepsin B
LigandBDBM16497
Substrate/CompetitorBDBM16498
Meas. Tech.Inhibitory Activity Measurement of Bovine Cathepsin B.
pH6±n/a
Temperature313.15±n/a K
IC50 24000±n/a nM
CommentsThe second order rate constant, k2 =1250 M-1s-1.
Citation Watanabe DYamamoto ATomoo KMatsumoto KMurata MKitamura KIshida T Quantitative evaluation of each catalytic subsite of cathepsin B for inhibitory activity based on inhibitory activity-binding mode relationship of epoxysuccinyl inhibitors by X-ray crystal structure analyses of complexes. J Mol Biol 362:979-93 (2006) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Cathepsin B
Name:Cathepsin B
Synonyms:BCSB | Cathepsin B precursor | Cathepsin B1
Type:Enzyme
Mol. Mass.:36657.87
Organism:Bos taurus (bovine)
Description:Bovine spleen CB was purchased from Sigma Co. (USA).
Residue:335
Sequence:
MWRLLATLSCLLVLTSARSSLYFPPLSDELVNFVNKQNTTWKAGHNFYNVDLSYVKKLCG
AILGGPKLPQRDAFAADVVLPESFDAREQWPNCPTIKEIRDQGSCGSCWAFGAVEAISDR
ICIHSNGRVNVEVSAEDMLTCCGGECGDGCNGGFPSGAWNFWTKKGLVSGGLYNSHVGCR
PYSIPPCEHHVNGSRPPCTGEGDTPKCSKTCEPGYSPSYKEDKHFGCSSYSVANNEKEIM
AEIYKNGPVEGAFSVYSDFLLYKSGVYQHVSGEIMGGHAIRILGWGVENGTPYWLVGNSW
NTDWGDNGFFKILRGQDHCGIESEIVAGMPCTHQY
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  Blast E-value cutoff:
BDBM16497
BDBM16498
NameBDBM16497
Synonyms:(2S,3S)-2-{[(2S,3S)-3-(ethoxycarbonyl)oxiran-2-yl]formamido}-3-methylpentanoic acid | CA inhibitor 1 | CA059 | EtO-tES-Ile-OH | N-{[(2S,3S)-3-(ethoxycarbonyl)oxiran-2-yl]carbonyl}-L-isoleucine | epoxysuccinyl derivative 1
TypeSmall organic molecule
Emp. Form.C12H19NO6
Mol. Mass.273.2824
SMILES[H][C@@]1(O[C@]1([H])C(=O)OCC)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O |r|
Structure
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