Ki Summary

Reaction Details

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Target

Cathepsin B

Ligand

BDBM16500

Substrate/Competitor

BDBM16498

Meas. Tech.

Inhibitory Activity Measurement of Bovine Cathepsin B.

6±n/a

Temperature

313.15±n/a K

IC50

24±n/a nM

Comments

The second order rate constant, k2 =153000 M-1s-1.

Citation

Watanabe, D; Yamamoto, A; Tomoo, K; Matsumoto, K; Murata, M; Kitamura, K; Ishida, T Quantitative evaluation of each catalytic subsite of cathepsin B for inhibitory activity based on inhibitory activity-binding mode relationship of epoxysuccinyl inhibitors by X-ray crystal structure analyses of complexes. J Mol Biol362:979-93 (2006) [PubMed] Article

More Info.:

Get all data from this article, Solution Info, Assay Method

Cathepsin B

Name:

Cathepsin B

Synonyms:

BCSB | CATB_BOVIN | CTSB | Cathepsin B precursor | Cathepsin B1

Type:

Enzyme

Mol. Mass.:

36657.87

Organism:

Bos taurus (bovine)

Description:

Bovine spleen CB was purchased from Sigma Co. (USA).

Residue:

335

Sequence:

MWRLLATLSCLLVLTSARSSLYFPPLSDELVNFVNKQNTTWKAGHNFYNVDLSYVKKLCG
AILGGPKLPQRDAFAADVVLPESFDAREQWPNCPTIKEIRDQGSCGSCWAFGAVEAISDR
ICIHSNGRVNVEVSAEDMLTCCGGECGDGCNGGFPSGAWNFWTKKGLVSGGLYNSHVGCR
PYSIPPCEHHVNGSRPPCTGEGDTPKCSKTCEPGYSPSYKEDKHFGCSSYSVANNEKEIM
AEIYKNGPVEGAFSVYSDFLLYKSGVYQHVSGEIMGGHAIRILGWGVENGTPYWLVGNSW
NTDWGDNGFFKILRGQDHCGIESEIVAGMPCTHQY

BDBM16500

BDBM16498

Name

BDBM16500

Synonyms:

(2S)-2-[(2S,3S)-2-{[(2S,3S)-3-(ethoxycarbonyl)oxiran-2-yl]formamido}-3-methylpentanamido]-3-methylpentanoic acid | CA inhibitor 3 | CA076 | EtO-tES-Ile-Ile-OH | N-{[(2S,3S)-3-(ethoxycarbonyl)oxiran-2-yl]carbonyl}-L-isoleucyl-L-isoleucine | epoxysuccinyl derivative 3

Type

Small organic molecule

Emp. Form.

C18H30N2O7

Mol. Mass.

386.44

SMILES

[H][C@@]1(O[C@]1([H])C(=O)OCC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)CC)C(O)=O |r|

Structure