Reaction Details |
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Target | 3-phosphoinositide-dependent protein kinase 1 [51-359,A162V] |
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Ligand | BDBM2691 |
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Substrate/Competitor | PDKtide |
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Meas. Tech. | PDK1 Kinase Activity Assay |
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pH | 7.5±n/a |
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Temperature | 303.15±n/a K |
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IC50 | 3400±n/a nM |
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Citation | Komander, D; Kular, GS; Schüttelkopf, AW; Deak, M; Prakash, KR; Bain, J; Elliott, M; Garrido-Franco, M; Kozikowski, AP; Alessi, DR; van Aalten, DM Interactions of LY333531 and other bisindolyl maleimide inhibitors with PDK1. Structure12:215-26 (2004) [PubMed] Article |
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More Info.: | Get all data from this article, Solution Info, Assay Method |
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3-phosphoinositide-dependent protein kinase 1 [51-359,A162V] |
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Name: | 3-phosphoinositide-dependent protein kinase 1 [51-359,A162V] |
Synonyms: | 3-Phosphoinositide-Dependent Protein Kinase 1 (PDK1) Mutant (A162V) | 3-phosphoinositide-dependent protein kinase 1 | PDK1 | PDPK1 | PDPK1_HUMAN | hPDK1 |
Type: | Kinase domain |
Mol. Mass.: | 35330.31 |
Organism: | Homo sapiens (Human) |
Description: | The mutant enzyme was generated using site-directed mutagenesis and expressed as glutathione-S-transferase (GST) fusion proteins in insect cells. |
Residue: | 309 |
Sequence: | MDGTAAEPRPGAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYAI
KILEKRHIIKENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKLYFGLSYVKNGELLKY
IRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPENILLNEDMHIQITDFGTAKVL
SPESKQARANSFVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLI
FQKIIKLEYDFPEKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENL
HQQTPPKLT
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BDBM2691 |
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PDKtide |
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Name: | PDKtide |
Synonyms: | Synthetic PDK substrate peptide |
Type: | Peptide |
Mol. Mass.: | 4766.24 |
Organism: | n/a |
Description: | n/a |
Residue: | 39 |
Sequence: | KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC
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