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TargetGag-Pol polyprotein [600-1029,C879S]/[600-1159,C879S]
LigandBDBM17673
Substrate/Competitorpoly(rC)-oligo(dG)
Meas. Tech.HIV-1 RT Enzyme Assay
pH8±n/a
Temperature310.15±n/a K
IC50 1610±n/a nM
Citation Morningstar, ML; Roth, T; Farnsworth, DW; Smith, MK; Watson, K; Buckheit, RW; Das, K; Zhang, W; Arnold, E; Julias, JG; Hughes, SH; Michejda, CJ Synthesis, Biological Activity, and Crystal Structure of Potent Nonnucleoside Inhibitors of HIV-1 Reverse Transcriptase That Retain Activity against Mutant Forms of the Enzyme. J Med Chem50:4003-4015 (2007) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Gag-Pol polyprotein [600-1029,C879S]/[600-1159,C879S]
Name:Gag-Pol polyprotein [600-1029,C879S]/[600-1159,C879S]
Synonyms:HIV-1 Reverse Transcriptase
Type:Protein Complex
Mol. Mass.:n/a
Description:Wild-type p66/p51 HIV-1 RT, derived from BH-10 clone of HIV-1, was expressed in bacteria.
Components:This complex has 2 components.
Component 1
Name:Gag-Pol polyprotein [600-1159,C879S]
Synonyms:HIV-1 Reverse Transcriptase Chain A | POL_HV1B1 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:64425.43
Organism:Human immunodeficiency virus type 1
Description:P03366[600-1159,C879S]
Residue:560
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTTNQKTELQ
AIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHK
GIGGNEQVDKLVSAGIRKIL
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Component 2
Name:Gag-Pol polyprotein [600-1029,C879S]
Synonyms:HIV-1 Reverse Transcriptase Chain B | POL_HV1B1 | gag-pol
Type:Enzyme Subunit
Mol. Mass.:50221.85
Organism:Human immunodeficiency virus type 1
Description:P03366[600-1029,C879S]
Residue:430
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL
DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI
YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT
VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP
PLVKLWYQLE
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BDBM17673
poly(rC)-oligo(dG)
Name:poly(rC)-oligo(dG)
Synonyms:n/a
Type:RNA-DNA duplex
Mol. Mass.:358.43
Organism:n/a
Description:The incorporation of Tritium-labeled [3H]dGTP onto a (poly)rC:(oligo)dG template:primer.
Residue:3
Sequence:
n/a