| Reaction Details |
|---|
 | Report a problem with these data |
| Target | Dihydrofolate reductase |
|---|
| Ligand | BDBM18251 |
|---|
| Substrate/Competitor | BDBM18044 |
|---|
| Meas. Tech. | Dihydrofolate Reductase (DHFR) Assay |
|---|
| pH | 7.4±n/a |
|---|
| Temperature | 303.15±n/a K |
|---|
| IC50 | 160±n/a nM |
|---|
| Citation |  Gangjee, A; Zeng, Y; Talreja, T; McGuire, JJ; Kisliuk, RL; Queener, SF Design and Synthesis of Classical and Nonclassical 6-Arylthio-2,4-diamino-5-ethylpyrrolo[2,3-d]pyrimidines as Antifolates. J Med Chem50:3046-3053 (2007) [PubMed] Article |
|---|
| More Info.: | Get all data from this article, Solution Info, Assay Method |
|---|
| |
| Dihydrofolate reductase |
|---|
| Name: | Dihydrofolate reductase |
|---|
| Synonyms: | Bacterial dihydrofolate reductase | DYR_ECOLI | Dihydrofolate Reductase (DHFR) | Tetrahydrofolate dehydrogenase | folA | tmrA |
|---|
| Type: | Enzyme |
|---|
| Mol. Mass.: | 17991.61 |
|---|
| Organism: | Escherichia coli |
|---|
| Description: | E. coli DHFR was expressed in BL21, and purified to homogeneity. |
|---|
| Residue: | 159 |
|---|
| Sequence: | MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNI
ILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVE
GDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR
|
|
|
|---|
| BDBM18251 |
|---|
| BDBM18044 |
|---|
| Name | BDBM18251 |
|---|
| Synonyms: | 6-[(3-chlorophenyl)sulfanyl]-5-ethyl-7H-pyrrolo[2,3-d]pyrimidine-2,4-diamine | Pyrrolo[2, 3-d]pyrimidine analogue, 7 |
|---|
| Type | Small organic molecule |
|---|
| Emp. Form. | C14H14ClN5S |
|---|
| Mol. Mass. | 319.812 |
|---|
| SMILES | CCc1c(Sc2cccc(Cl)c2)[nH]c2nc(N)nc(N)c12 |
|---|
| Structure | 
|
|---|
Search and Browse
