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TargetDihydrofolate Reductase (DHFR)
LigandBDBM18050
Substrate/CompetitorBDBM18044
Meas. Tech.In Vitro Inhibition Assay
pH7±n/a
Temperature298.15±n/a K
IC50 24±21 nM
Citation Joska TMAnderson AC Structure-activity relationships of Bacillus cereus and Bacillus anthracis dihydrofolate reductase: toward the identification of new potent drug leads. Antimicrob Agents Chemother 50:3435-43 (2006) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Dihydrofolate Reductase (DHFR)
Name:Dihydrofolate Reductase (DHFR)
Synonyms:Tetrahydrofolate dehydrogenase
Type:Enzyme
Mol. Mass.:19144.66
Organism:Bacillus cereus (ATCC 14579)
Description:Q81E04
Residue:162
Sequence:
MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRN
IIVTRNEGYHVEGCEVVHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHA
FEGDTFFPEIDMTNWKEIFVEKGLTDEKNPYTYYYHVYEKQQ
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  Blast E-value cutoff:
BDBM18050
BDBM18044
NameBDBM18050
Synonyms:2-[(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)amino}phenyl)formamido]pentanedioic acid | CHEMBL34259 | METHOTREXATE | MTX | Methotrexate | cid_126941
TypeSmall organic molecule
Emp. Form.C20H22N8O5
Mol. Mass.454.4393
SMILESCN(Cc1cnc2nc(N)nc(N)c2n1)c1ccc(cc1)C(=O)N[C@@H](CCC(O)=O)C(O)=O |r|
Structure
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